Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures

Badri Lakshmanrao Aekbote, Jaroslaw Jacak, Gerhard J. Schütz, Erzsébet Csányi, Zsolt Szegletes, P. Ormos, L. Kelemen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

There is an increasing interest in functionalized complex 3D microstructures with sub-micrometer features for micro- and nanotechnology applications in biology. Depending primarily on the material of the structures various methods exist to create functional layers of simple chemical groups, biological macromolecules or metal nanoparticles. Here an effective coating method is demonstrated and evaluated on SU-8 based 3D microstructures made by two-photon polymerization. Protein streptavidin and gold nanoparticles (NP) were bound to the microstructures utilizing acid treatment-mediated silane chemistry. The protein surface density, quantified with single molecule fluorescence microscopy revealed that the protein forms a third of a monolayer on the two-photon polymerized structures. The surface coverage of the gold NPs on the microstructures was simply controlled with a single parameter. The possible degrading effect of the acid treatment on the sub-micrometer features of the TPP microstructures was analyzed. Our results show that the silane chemistry-based method, used earlier for the functionalization of large-area surfaces can effectively be adapted to coat two-photon polymerized SU-8 microstructures with sub-micrometer features.

Original languageEnglish
Pages (from-to)1745-1754
Number of pages10
JournalEuropean Polymer Journal
Volume48
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Photons
microstructure
Microstructure
photons
Silanes
micrometers
proteins
Proteins
silanes
Gold
chemistry
gold
nanoparticles
acids
Acids
Streptavidin
Metal nanoparticles
Fluorescence microscopy
nanotechnology
Macromolecules

Keywords

  • 3D microstructures
  • Aminosilane treatment
  • Gold nanoparticle
  • Optical trap
  • Surface functionalization
  • Two-photon polymerization

ASJC Scopus subject areas

  • Polymers and Plastics
  • Physics and Astronomy(all)
  • Organic Chemistry

Cite this

Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures. / Aekbote, Badri Lakshmanrao; Jacak, Jaroslaw; Schütz, Gerhard J.; Csányi, Erzsébet; Szegletes, Zsolt; Ormos, P.; Kelemen, L.

In: European Polymer Journal, Vol. 48, No. 10, 10.2012, p. 1745-1754.

Research output: Contribution to journalArticle

Aekbote, Badri Lakshmanrao ; Jacak, Jaroslaw ; Schütz, Gerhard J. ; Csányi, Erzsébet ; Szegletes, Zsolt ; Ormos, P. ; Kelemen, L. / Aminosilane-based functionalization of two-photon polymerized 3D SU-8 microstructures. In: European Polymer Journal. 2012 ; Vol. 48, No. 10. pp. 1745-1754.
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