Aminoguanidine reduces brain lesion volume after cold injury in the rat

C. Görlach, T. Hortobágyi, Z. Benyó, M. Wahl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The aim of this study was to examine the effect of aminoguanidine (AG), which is thought to be an inducible nitric oxide synthase (iNOS) inhibitor, on lesion volume induced by cold injury in the parietal cortex of the rat. Cold lesion was induced by applying a precooled (-78°C) copper cylinder (diameter: 3 mm) for 6 s to the intact dura. Lesion volume was determined using the triphenyltetrazolium-chloride method after 24 h. Pretreatment (1 h) and posttreatment (7.5 h) with AG [10 or 100 mg/kg body mass (BM)] reduced the lesion volume by 15 and 27%, respectively. However, posttreatment alone with AG (10 and 100 mg/kg BM) caused less of a reduction in lesion volume, by 8 and 20%, respectively. Pre- and posttreatment with AG also reduced the plasma nitrate/nitrite concentration compared with lesioned, saline-treated rats. Only a double therapy with AG (100 mg/kg BM) resulted in a significant reduction (48%) compared to saline alone, which was even larger (55%) compared to the sham group. The tissue nitrate/nitrite concentration was significantly attenuated by pre- and posttreatment with AG (100 mg/kg BM) not only in the ipsilateral but also in the contralateral hemisphere. There was no difference regarding the parameter between shams and lesioned, saline- treated rats. Since combined pre- and posttreatment with AG reduced the lesion volume more than posttreatment alone and the plasma and tissue nitrate/nitrite concentrations were diminished during AG therapy compared to shams, we hypothesize that AG inhibits hot only iNOS but also other enzymes, such as nNOS, diamine oxidase, and advanced glycation endproducts synthase.

Original languageEnglish
Pages (from-to)309-314
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Volume440
Issue number2
DOIs
Publication statusPublished - 2000

Fingerprint

Rats
Brain
Nitrites
Nitrates
Nitric Oxide Synthase Type II
Tissue
Amine Oxidase (Copper-Containing)
Plasmas
pimagedine
Cold Injury
Parietal Lobe
Copper
Enzymes
Therapeutics

Keywords

  • Cold lesion
  • Inducible nitric oxide synthase
  • Lesion volume
  • Neuronal nitric oxide synthase
  • Nitrate/nitrite concentration

ASJC Scopus subject areas

  • Physiology

Cite this

Aminoguanidine reduces brain lesion volume after cold injury in the rat. / Görlach, C.; Hortobágyi, T.; Benyó, Z.; Wahl, M.

In: Pflugers Archiv European Journal of Physiology, Vol. 440, No. 2, 2000, p. 309-314.

Research output: Contribution to journalArticle

@article{221c3e0e04974a60b8729df5f38ee1dc,
title = "Aminoguanidine reduces brain lesion volume after cold injury in the rat",
abstract = "The aim of this study was to examine the effect of aminoguanidine (AG), which is thought to be an inducible nitric oxide synthase (iNOS) inhibitor, on lesion volume induced by cold injury in the parietal cortex of the rat. Cold lesion was induced by applying a precooled (-78°C) copper cylinder (diameter: 3 mm) for 6 s to the intact dura. Lesion volume was determined using the triphenyltetrazolium-chloride method after 24 h. Pretreatment (1 h) and posttreatment (7.5 h) with AG [10 or 100 mg/kg body mass (BM)] reduced the lesion volume by 15 and 27{\%}, respectively. However, posttreatment alone with AG (10 and 100 mg/kg BM) caused less of a reduction in lesion volume, by 8 and 20{\%}, respectively. Pre- and posttreatment with AG also reduced the plasma nitrate/nitrite concentration compared with lesioned, saline-treated rats. Only a double therapy with AG (100 mg/kg BM) resulted in a significant reduction (48{\%}) compared to saline alone, which was even larger (55{\%}) compared to the sham group. The tissue nitrate/nitrite concentration was significantly attenuated by pre- and posttreatment with AG (100 mg/kg BM) not only in the ipsilateral but also in the contralateral hemisphere. There was no difference regarding the parameter between shams and lesioned, saline- treated rats. Since combined pre- and posttreatment with AG reduced the lesion volume more than posttreatment alone and the plasma and tissue nitrate/nitrite concentrations were diminished during AG therapy compared to shams, we hypothesize that AG inhibits hot only iNOS but also other enzymes, such as nNOS, diamine oxidase, and advanced glycation endproducts synthase.",
keywords = "Cold lesion, Inducible nitric oxide synthase, Lesion volume, Neuronal nitric oxide synthase, Nitrate/nitrite concentration",
author = "C. G{\"o}rlach and T. Hortob{\'a}gyi and Z. Beny{\'o} and M. Wahl",
year = "2000",
doi = "10.1007/s004240051053",
language = "English",
volume = "440",
pages = "309--314",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Aminoguanidine reduces brain lesion volume after cold injury in the rat

AU - Görlach, C.

AU - Hortobágyi, T.

AU - Benyó, Z.

AU - Wahl, M.

PY - 2000

Y1 - 2000

N2 - The aim of this study was to examine the effect of aminoguanidine (AG), which is thought to be an inducible nitric oxide synthase (iNOS) inhibitor, on lesion volume induced by cold injury in the parietal cortex of the rat. Cold lesion was induced by applying a precooled (-78°C) copper cylinder (diameter: 3 mm) for 6 s to the intact dura. Lesion volume was determined using the triphenyltetrazolium-chloride method after 24 h. Pretreatment (1 h) and posttreatment (7.5 h) with AG [10 or 100 mg/kg body mass (BM)] reduced the lesion volume by 15 and 27%, respectively. However, posttreatment alone with AG (10 and 100 mg/kg BM) caused less of a reduction in lesion volume, by 8 and 20%, respectively. Pre- and posttreatment with AG also reduced the plasma nitrate/nitrite concentration compared with lesioned, saline-treated rats. Only a double therapy with AG (100 mg/kg BM) resulted in a significant reduction (48%) compared to saline alone, which was even larger (55%) compared to the sham group. The tissue nitrate/nitrite concentration was significantly attenuated by pre- and posttreatment with AG (100 mg/kg BM) not only in the ipsilateral but also in the contralateral hemisphere. There was no difference regarding the parameter between shams and lesioned, saline- treated rats. Since combined pre- and posttreatment with AG reduced the lesion volume more than posttreatment alone and the plasma and tissue nitrate/nitrite concentrations were diminished during AG therapy compared to shams, we hypothesize that AG inhibits hot only iNOS but also other enzymes, such as nNOS, diamine oxidase, and advanced glycation endproducts synthase.

AB - The aim of this study was to examine the effect of aminoguanidine (AG), which is thought to be an inducible nitric oxide synthase (iNOS) inhibitor, on lesion volume induced by cold injury in the parietal cortex of the rat. Cold lesion was induced by applying a precooled (-78°C) copper cylinder (diameter: 3 mm) for 6 s to the intact dura. Lesion volume was determined using the triphenyltetrazolium-chloride method after 24 h. Pretreatment (1 h) and posttreatment (7.5 h) with AG [10 or 100 mg/kg body mass (BM)] reduced the lesion volume by 15 and 27%, respectively. However, posttreatment alone with AG (10 and 100 mg/kg BM) caused less of a reduction in lesion volume, by 8 and 20%, respectively. Pre- and posttreatment with AG also reduced the plasma nitrate/nitrite concentration compared with lesioned, saline-treated rats. Only a double therapy with AG (100 mg/kg BM) resulted in a significant reduction (48%) compared to saline alone, which was even larger (55%) compared to the sham group. The tissue nitrate/nitrite concentration was significantly attenuated by pre- and posttreatment with AG (100 mg/kg BM) not only in the ipsilateral but also in the contralateral hemisphere. There was no difference regarding the parameter between shams and lesioned, saline- treated rats. Since combined pre- and posttreatment with AG reduced the lesion volume more than posttreatment alone and the plasma and tissue nitrate/nitrite concentrations were diminished during AG therapy compared to shams, we hypothesize that AG inhibits hot only iNOS but also other enzymes, such as nNOS, diamine oxidase, and advanced glycation endproducts synthase.

KW - Cold lesion

KW - Inducible nitric oxide synthase

KW - Lesion volume

KW - Neuronal nitric oxide synthase

KW - Nitrate/nitrite concentration

UR - http://www.scopus.com/inward/record.url?scp=0006223390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0006223390&partnerID=8YFLogxK

U2 - 10.1007/s004240051053

DO - 10.1007/s004240051053

M3 - Article

C2 - 10898532

AN - SCOPUS:0006223390

VL - 440

SP - 309

EP - 314

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 2

ER -