Amino acid cluster formation studied by electrospray ionization mass spectrometry

Péter Nemes, G. Schlosser, K. Vékey

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The association properties of natural and non-natural amino acids were studied in detail using electrospray ionization mass spectrometry. The results show a highly diverse cluster formation behavior of amino acids. There are differences regarding the degree of clustering (average cluster size), the presence or absence of one or several 'magic' clusters of special stability and the influence of chirality on cluster stability. Cluster formation does not show a good correlation with simple physico-chemical properties (such as solubility), indicating that it is a specific process and not only a simple aggregation during evaporation/ionization. A systematic study of cluster formation of serine derivatives reveals that all functional groups play a prominent role in the binding of the magic octamer. The results support the idea of the zwitterionic character of the octamer. Electrospray ionization of the side-chain acetylated serine shows the formation of a very stable tetramer with a strong preference for homochirality. The results suggest that Ser8 is made up of two tetramer subunits, held together by hydrogen bonds of the side-chain.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalJournal of Mass Spectrometry
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 2005

Fingerprint

Electrospray ionization
Magic
Electrospray Ionization Mass Spectrometry
Serine
Mass spectrometry
amino acids
Amino acids
mass spectroscopy
Amino Acids
ionization
Chirality
Solubility
Chemical properties
Functional groups
Ionization
Cluster Analysis
Hydrogen
Hydrogen bonds
Evaporation
Agglomeration

Keywords

  • Amino acid
  • Chiral discrimination
  • Cluster
  • Magic number
  • Serine

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

Cite this

Amino acid cluster formation studied by electrospray ionization mass spectrometry. / Nemes, Péter; Schlosser, G.; Vékey, K.

In: Journal of Mass Spectrometry, Vol. 40, No. 1, 01.2005, p. 43-49.

Research output: Contribution to journalArticle

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