Aminated single-walled carbon nanotubes as carrier for covalent immobilization of phenylalanine ammonia-lyase

Judith Hajnal Bartha-Vári, László Csaba Bencze, Evelin Bell, L. Poppe, Gabriel Katona, Florin Dan Irimie, Csaba Paizs, Monica Ioana Toşa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new and efficient immobilized form of phenylalanine ammonia-lyase (PAL) was obtained by covalent linkage onto amino functionalized single-walled carbon nanotubes (SwCNTNH2) as carrier. The catalytic properties of the resulted nanostructured biocatalyst (SwCNTNH2-PAL) were tested in the kinetic resolution of racemic 2-amino-3-(thiophen-2-yl)propanoic acid 1 by ammonia elimination and in the enantiotope selective addition of ammonia onto (E)-3-(thiophen-2-yl)acrylic acid 2. SwCNTNH2-PAL was a durable biocatalyst in batch mode for ammonia elimination from 1 (>85% of original activity after 7 cycles) and in ammonia addition to 2 (>70% of original activity after 3 cycles in 6 M NH3, pH 10.0). The ammonia addition onto 2 was also studied in a continuous-flow microreactor packed with SwCNTNH2-PAL (2 M NH3, pH 10.0, 15 bar) in the 30-80°C temperature range. No significant loss of PAL activity was observed over 72 h in the microreactor up to 60 °C. Productivity of SwCNTNH2-PAL at 30 °C was significantly higher in the enantiotope selective ammonia addition performed in a packed-bed reactor operated in continuous-flow mode (rflow = 2.41 mmol min-1 g-1) than in the reaction performed in batch system (rbatch= 1.38 mmol min-1 g-1).

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalPeriodica Polytechnica: Chemical Engineering
Volume61
Issue number1
DOIs
Publication statusPublished - 2017

Fingerprint

Phenylalanine Ammonia-Lyase
Single-walled carbon nanotubes (SWCN)
Ammonia
Biocatalysts
Packed beds
Enzymes
Productivity
Kinetics
Acids
Acrylics

Keywords

  • Aminated SwCNT
  • Continuous-flow system
  • Covalent immobilization
  • PAL

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Aminated single-walled carbon nanotubes as carrier for covalent immobilization of phenylalanine ammonia-lyase. / Bartha-Vári, Judith Hajnal; Bencze, László Csaba; Bell, Evelin; Poppe, L.; Katona, Gabriel; Irimie, Florin Dan; Paizs, Csaba; Toşa, Monica Ioana.

In: Periodica Polytechnica: Chemical Engineering, Vol. 61, No. 1, 2017, p. 59-66.

Research output: Contribution to journalArticle

Bartha-Vári, Judith Hajnal ; Bencze, László Csaba ; Bell, Evelin ; Poppe, L. ; Katona, Gabriel ; Irimie, Florin Dan ; Paizs, Csaba ; Toşa, Monica Ioana. / Aminated single-walled carbon nanotubes as carrier for covalent immobilization of phenylalanine ammonia-lyase. In: Periodica Polytechnica: Chemical Engineering. 2017 ; Vol. 61, No. 1. pp. 59-66.
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