Alternative ultrastructural localization of Dolichos biflorus lectin binding sites in proton secreting parietal cells of mice

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Abstract

High amount of N-acetyl-d-galactosamine specific lectin binding sites were detected on the canalicular membranes of human parietal cells. Our present model investigations on mice showed that the intracellular distribution of the terminal N-acetyl-d-galactosamine containing glycoprotein highly depends on the actual functional state of the parietal cells. In the normal gastric mucosa 40%-60% of parictal cells react positively after staining with horseradish peroxidase or biotin labelled Dolichos biflorus lectin. Ultrastructurally lectin binding sites occur mainly on the basolateral membrane infoldings in fed animals, while they are present exclusively on the canalicular membranes of fasting mice, suggesting that the alternative appearance of lectin binding sites on the opposite membrane areas of parietal cells is tightly coupled to their main function, to H+ secretion.

Original languageEnglish
Pages (from-to)479-482
Number of pages4
JournalHistochemistry
Volume87
Issue number5
DOIs
Publication statusPublished - Sep 1987

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Protons
Binding Sites
Lectins
Galactosamine
Membranes
Horseradish Peroxidase
Biotin
Gastric Mucosa
Fasting
Glycoproteins
Staining and Labeling
dolichos biflorus agglutinin

ASJC Scopus subject areas

  • Anatomy
  • Medicine(all)

Cite this

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title = "Alternative ultrastructural localization of Dolichos biflorus lectin binding sites in proton secreting parietal cells of mice",
abstract = "High amount of N-acetyl-d-galactosamine specific lectin binding sites were detected on the canalicular membranes of human parietal cells. Our present model investigations on mice showed that the intracellular distribution of the terminal N-acetyl-d-galactosamine containing glycoprotein highly depends on the actual functional state of the parietal cells. In the normal gastric mucosa 40{\%}-60{\%} of parictal cells react positively after staining with horseradish peroxidase or biotin labelled Dolichos biflorus lectin. Ultrastructurally lectin binding sites occur mainly on the basolateral membrane infoldings in fed animals, while they are present exclusively on the canalicular membranes of fasting mice, suggesting that the alternative appearance of lectin binding sites on the opposite membrane areas of parietal cells is tightly coupled to their main function, to H+ secretion.",
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