Alternative cyanide-sensitive oxidase interacting with photosynthesis in Synechocystis PCC6803. Ancestor of the terminal oxidase of chlororespiration?

C. Büchel, O. Zsíros, G. Garab

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Influence of respiration on photosynthesis in Synechocystis PCC6803 was studied by measuring the redox transients of cytochrome f (cyt f) upon excitation of the cells with repetitive single turnover flashes. Upon the addition of KCN the flash-induced oxidation of cyt f was increased and the rereduction of cyt f+ was accelerated. Dependence of these effects on the concentration of KCN clearly demonstrated the existence of two cyanide-sensitive oxidases interacting with photosynthesis: cyt aa3, which was sensitive to low concentrations of cyanide, and an alternative oxidase, which could be suppressed by using ≥1 mM KCN. The interaction between the photosynthetic and the respiratory electron transport chains was regulated mainly by the activity of the alternative cyanide-sensitive oxidase. The oxidative pathway involving the alternative cyanide-sensitive oxidase was insensitive to salicyl hydroxamic acid and azide. The close resemblance of the inhibition pattern reported here and that described for chlororespiration in algae and higher plants strongly suggest that an oxidase of the same type as the alternative cyanide-sensitive oxidase of cyanobacteria functions as a terminal oxidase in chloroplasts.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalPhotosynthetica
Volume35
Issue number2
DOIs
Publication statusPublished - 1998

Fingerprint

Synechocystis
Photosynthesis
cyanides
Cytochromes f
cytochrome f
Oxidoreductases
ancestry
photosynthesis
algae
Respiratory Transport
hydroxamic acids
Hydroxamic Acids
azides
Azides
electron transport chain
Cyanobacteria
Chloroplasts
Electron Transport
cell respiration
Oxidation-Reduction

Keywords

  • Absorbance
  • Azide
  • Cyanobacteria
  • Cytochromes
  • Flash irradiation
  • KCN
  • NaN
  • Phylogeny
  • Respiration
  • Salicyl hydroxamic acid

ASJC Scopus subject areas

  • Plant Science

Cite this

Alternative cyanide-sensitive oxidase interacting with photosynthesis in Synechocystis PCC6803. Ancestor of the terminal oxidase of chlororespiration? / Büchel, C.; Zsíros, O.; Garab, G.

In: Photosynthetica, Vol. 35, No. 2, 1998, p. 223-231.

Research output: Contribution to journalArticle

@article{e824789434d34e76bc980055558fc9ea,
title = "Alternative cyanide-sensitive oxidase interacting with photosynthesis in Synechocystis PCC6803. Ancestor of the terminal oxidase of chlororespiration?",
abstract = "Influence of respiration on photosynthesis in Synechocystis PCC6803 was studied by measuring the redox transients of cytochrome f (cyt f) upon excitation of the cells with repetitive single turnover flashes. Upon the addition of KCN the flash-induced oxidation of cyt f was increased and the rereduction of cyt f+ was accelerated. Dependence of these effects on the concentration of KCN clearly demonstrated the existence of two cyanide-sensitive oxidases interacting with photosynthesis: cyt aa3, which was sensitive to low concentrations of cyanide, and an alternative oxidase, which could be suppressed by using ≥1 mM KCN. The interaction between the photosynthetic and the respiratory electron transport chains was regulated mainly by the activity of the alternative cyanide-sensitive oxidase. The oxidative pathway involving the alternative cyanide-sensitive oxidase was insensitive to salicyl hydroxamic acid and azide. The close resemblance of the inhibition pattern reported here and that described for chlororespiration in algae and higher plants strongly suggest that an oxidase of the same type as the alternative cyanide-sensitive oxidase of cyanobacteria functions as a terminal oxidase in chloroplasts.",
keywords = "Absorbance, Azide, Cyanobacteria, Cytochromes, Flash irradiation, KCN, NaN, Phylogeny, Respiration, Salicyl hydroxamic acid",
author = "C. B{\"u}chel and O. Zs{\'i}ros and G. Garab",
year = "1998",
doi = "10.1023/A:1006958706540",
language = "English",
volume = "35",
pages = "223--231",
journal = "Photosynthetica",
issn = "0300-3604",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - Alternative cyanide-sensitive oxidase interacting with photosynthesis in Synechocystis PCC6803. Ancestor of the terminal oxidase of chlororespiration?

AU - Büchel, C.

AU - Zsíros, O.

AU - Garab, G.

PY - 1998

Y1 - 1998

N2 - Influence of respiration on photosynthesis in Synechocystis PCC6803 was studied by measuring the redox transients of cytochrome f (cyt f) upon excitation of the cells with repetitive single turnover flashes. Upon the addition of KCN the flash-induced oxidation of cyt f was increased and the rereduction of cyt f+ was accelerated. Dependence of these effects on the concentration of KCN clearly demonstrated the existence of two cyanide-sensitive oxidases interacting with photosynthesis: cyt aa3, which was sensitive to low concentrations of cyanide, and an alternative oxidase, which could be suppressed by using ≥1 mM KCN. The interaction between the photosynthetic and the respiratory electron transport chains was regulated mainly by the activity of the alternative cyanide-sensitive oxidase. The oxidative pathway involving the alternative cyanide-sensitive oxidase was insensitive to salicyl hydroxamic acid and azide. The close resemblance of the inhibition pattern reported here and that described for chlororespiration in algae and higher plants strongly suggest that an oxidase of the same type as the alternative cyanide-sensitive oxidase of cyanobacteria functions as a terminal oxidase in chloroplasts.

AB - Influence of respiration on photosynthesis in Synechocystis PCC6803 was studied by measuring the redox transients of cytochrome f (cyt f) upon excitation of the cells with repetitive single turnover flashes. Upon the addition of KCN the flash-induced oxidation of cyt f was increased and the rereduction of cyt f+ was accelerated. Dependence of these effects on the concentration of KCN clearly demonstrated the existence of two cyanide-sensitive oxidases interacting with photosynthesis: cyt aa3, which was sensitive to low concentrations of cyanide, and an alternative oxidase, which could be suppressed by using ≥1 mM KCN. The interaction between the photosynthetic and the respiratory electron transport chains was regulated mainly by the activity of the alternative cyanide-sensitive oxidase. The oxidative pathway involving the alternative cyanide-sensitive oxidase was insensitive to salicyl hydroxamic acid and azide. The close resemblance of the inhibition pattern reported here and that described for chlororespiration in algae and higher plants strongly suggest that an oxidase of the same type as the alternative cyanide-sensitive oxidase of cyanobacteria functions as a terminal oxidase in chloroplasts.

KW - Absorbance

KW - Azide

KW - Cyanobacteria

KW - Cytochromes

KW - Flash irradiation

KW - KCN

KW - NaN

KW - Phylogeny

KW - Respiration

KW - Salicyl hydroxamic acid

UR - http://www.scopus.com/inward/record.url?scp=0032417441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032417441&partnerID=8YFLogxK

U2 - 10.1023/A:1006958706540

DO - 10.1023/A:1006958706540

M3 - Article

AN - SCOPUS:0032417441

VL - 35

SP - 223

EP - 231

JO - Photosynthetica

JF - Photosynthetica

SN - 0300-3604

IS - 2

ER -