Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment

Nisha K. Ramakrishnan, Krisztina Marosi, C. Nyakas, Chantal Kwizera, Philip H. Elsinga, Kiichi Ishiwata, Paul G M Luiten, Rudi A J O Dierckx, Aren van Waarde

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: Sigma-1 receptors are involved in learning and memory processes. We assessed sigma-1 receptor expression and memory function in two animal models of cognitive impairment.Procedures: Male Wistar-Hannover rats were either lesioned by unilateral injection of N-methyl-D-aspartic acid in the nucleus basalis, or deprived of rapid eye movement sleep for 48 h, using the modified multiple platform method. Sigma-1 receptor expression was examined with the positron emission tomography radiotracer [11C]SA4503, immunohistochemistry, and Western blotting.Results: Cortical tracer uptake after 1 week was not significantly affected by lesioning. Immunohistochemistry revealed moderate increases of sigma-1 receptors at bregma level −2.8, in parietal cortex layer V of the lesioned hemisphere. Sleep deprivation lowered passive avoidance test scores and reduced [11C]SA4503 accumulation and sigma-1 receptor expression in pons.Conclusions: Cholinergic lesioning causes an increase of sigma-1 receptor expression in a small cortical area which may be neuroprotective. Sleep deprivation decreases receptor expression in midbrain and pons.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalMolecular Imaging and Biology
Volume17
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Animal Models
Sleep Deprivation
Pons
Immunohistochemistry
Parietal Lobe
REM Sleep
N-Methylaspartate
Mesencephalon
Positron-Emission Tomography
Cholinergic Agents
Cognitive Dysfunction
sigma-1 receptor
Wistar Rats
Sleep
Western Blotting
Learning
Injections
SA 4503

Keywords

  • Cholinergic lesion
  • microPET
  • Novel object recognition
  • Passive avoidance
  • REM sleep deprivation
  • Sigma-1 receptor
  • [<sup>11</sup>C]SA4503

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Ramakrishnan, N. K., Marosi, K., Nyakas, C., Kwizera, C., Elsinga, P. H., Ishiwata, K., ... van Waarde, A. (2015). Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment. Molecular Imaging and Biology, 17(2), 231-238. https://doi.org/10.1007/s11307-014-0780-x

Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment. / Ramakrishnan, Nisha K.; Marosi, Krisztina; Nyakas, C.; Kwizera, Chantal; Elsinga, Philip H.; Ishiwata, Kiichi; Luiten, Paul G M; Dierckx, Rudi A J O; van Waarde, Aren.

In: Molecular Imaging and Biology, Vol. 17, No. 2, 2015, p. 231-238.

Research output: Contribution to journalArticle

Ramakrishnan, NK, Marosi, K, Nyakas, C, Kwizera, C, Elsinga, PH, Ishiwata, K, Luiten, PGM, Dierckx, RAJO & van Waarde, A 2015, 'Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment', Molecular Imaging and Biology, vol. 17, no. 2, pp. 231-238. https://doi.org/10.1007/s11307-014-0780-x
Ramakrishnan, Nisha K. ; Marosi, Krisztina ; Nyakas, C. ; Kwizera, Chantal ; Elsinga, Philip H. ; Ishiwata, Kiichi ; Luiten, Paul G M ; Dierckx, Rudi A J O ; van Waarde, Aren. / Altered Sigma-1 Receptor Expression in Two Animal Models of Cognitive Impairment. In: Molecular Imaging and Biology. 2015 ; Vol. 17, No. 2. pp. 231-238.
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