Altered sialylation on the cell-surface proteins of dexamethasone-treated human macrophages contributes to augmented uptake of apoptotic neutrophils

A. Mádi, Gyöngyike Majai, Cornelia Koy, G. Vámosi, Attila Szántó, Michael O. Glocker, L. Fésüs

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Macrophages eliminate apoptotic granulocytes before their secondary necrosis during resolution of inflammation. A well-known glucocorticoid, the anti-inflammatory dexamethasone augments phagocytosis capacity of macrophages with a so far not fully clarified mechanism. We have found that sialylation of cell-surface proteins on human macrophages is markedly altered by dexamethasone. Compared to non-treated cells, dexamethasone-treated macrophages can bind significantly less Sambucus nigra lectin specific for sialic acids on their surfaces as a result of undersialylation of annexin-II and an HLA-II protein. Non-treated macrophages covered by S. nigra lectin had increased uptake of apoptotic cells; however, the significantly higher phagocytosis capacity of dexamethasone-treated macrophages could not be stimulated further this way. Our results suggest that dexamethasone treatment leads to decreased number of sialic acids on the surfaces of human macrophages promoting recognition and uptake of apoptotic cells.

Original languageEnglish
Pages (from-to)88-95
Number of pages8
JournalImmunology Letters
Volume135
Issue number1-2
DOIs
Publication statusPublished - Mar 30 2011

Fingerprint

Dexamethasone
Membrane Proteins
Neutrophils
Macrophages
Sialic Acids
Phagocytosis
Annexin A2
Granulocytes
Glucocorticoids
Anti-Inflammatory Agents
Necrosis
Inflammation
Proteins

Keywords

  • Annexin II
  • Apoptosis
  • Dexamethasone
  • Haptoglobin
  • Human macrophages
  • Programmed cell death

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Altered sialylation on the cell-surface proteins of dexamethasone-treated human macrophages contributes to augmented uptake of apoptotic neutrophils. / Mádi, A.; Majai, Gyöngyike; Koy, Cornelia; Vámosi, G.; Szántó, Attila; Glocker, Michael O.; Fésüs, L.

In: Immunology Letters, Vol. 135, No. 1-2, 30.03.2011, p. 88-95.

Research output: Contribution to journalArticle

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AU - Majai, Gyöngyike

AU - Koy, Cornelia

AU - Vámosi, G.

AU - Szántó, Attila

AU - Glocker, Michael O.

AU - Fésüs, L.

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