Altered postreceptor signal transduction of formyl-Met-Leu-Phe receptors in polymorphonuclear leukocytes of patients with non-insulin-dependent diabetes mellitus

Gabriella Fóris, G. Paragh, B. Dezső, Tamás Keresztes, Zoltán Balogh, Jenõ Szabó

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The signal transduction of the formyl-Met-Leu-Phe (FMLP) receptor in polymorphonuclear leukocytes (PMNLs) from patients with non-insulin-dependent diabetes mellitus (NIDDM) was compared to that of PMNLs obtained from healthy volunteers. According to our previous studies in this group of patients neither the decrease in insulin binding capacity nor the enhanced insulin- degrading enzyme activity was involved. In control PMNLs, 10 nM FMLP induced a pertussis toxin-sensitive increase in phosphatidyl inositol (PI) cleavage and a subsequent Ca2+ signaling from the intracellular pools. On the other hand, the FMLP-induced protein kinase C (PKC) activation and translocation into the membrane could not be detected in these cells via the measurement of 32P incorporation into histone. In contrast, in PMNLs of this special group of patients suffering from NIDDM the FMLP stimulus produced a significantly low increase in PI cleavage and Ca2+ signaling from the intracellular pools. Moreover, in resting PMNLs of these patients with NIDDM, not only the [Ca2+](i) but also the membrane-bound PKC activity was found to be significantly increased. In addition, PKC translocation into the cell membrane of diabetic PMNLs could be further triggered with FMLP as judged by the measurement of 32P incorporation into histone. Based on these results, it appears that the signaling of FMLP receptors in PMNLs of some NIDDM patients may have an alternative pathway through Ca2+ influx from extracellular medium, arachidonic acid cascade, and PKC activation.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalClinical Immunology and Immunopathology
Volume86
Issue number1
DOIs
Publication statusPublished - Jan 1998

Fingerprint

methionyl-leucyl-phenylalanine
Type 2 Diabetes Mellitus
Signal Transduction
Neutrophils
Protein Kinase C
Phosphatidylinositols
Histones
Insulysin
Pertussis Toxin
Arachidonic Acid
Healthy Volunteers
Membrane Proteins
Cell Membrane
Insulin
Membranes

Keywords

  • Diabetes mellitus
  • FMLP receptor
  • Leukocyte
  • Signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pathology and Forensic Medicine

Cite this

Altered postreceptor signal transduction of formyl-Met-Leu-Phe receptors in polymorphonuclear leukocytes of patients with non-insulin-dependent diabetes mellitus. / Fóris, Gabriella; Paragh, G.; Dezső, B.; Keresztes, Tamás; Balogh, Zoltán; Szabó, Jenõ.

In: Clinical Immunology and Immunopathology, Vol. 86, No. 1, 01.1998, p. 95-101.

Research output: Contribution to journalArticle

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