Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease

Nóra Judit Béres, Zoltán Kiss, Zsófia Sztupinszki, Gábor Lendvai, A. Arató, Erna Sziksz, Ádám Vannay, Attila J. Szabó, Katalin Eszter Müller, Áron Cseh, Kriszta Boros, G. Verès

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Abstract

Introduction MicroRNAs (miRs) came recently into focus as promising novel research targets offering new insights into the pathogenesis of inflammatory bowel diseases (IBD). Aims The aim of our study was to identify a pediatric IBD (pIBD) characteristic miR profile serving as potential Crohn's disease (CD) and ulcerative colitis (UC) specific diagnostic pattern and to further analyze the related target genes. Methods Small RNA sequencing was performed on inflamed and intact colonic biopsies of CD, and control patients. Selected miRs were further investigated by RT-PCR, complemented with an UC group, in order to address the differential diagnostic potential of miRs in the two IBD subtypes. To analyze network connection of differentially expressed miRs and their target genes MiRTarBase database and previous transcriptome sequencing data from pediatric patient groups were used. Results Sequencing analysis identified 170 miRs with altered expression. RT-PCR analysis revealed altered expression of miR-31, -125a, -142-3p, and -146a discriminating between the inflamed mucosa of CD and UC. In the intact mucosa of CD patients the expression of miR-18a, -20a, -21, -31, -99a, -99b, -100, -125a, -126, -142-5p, -146a, -185, -204, -221, and -223 was elevated compared to the controls. The expression of miR-20a, -204 and -221 was elevated exclusively in the intact region of CD patients compared to the controls. Enrichment analysis identified main IBD-related functional groups. Conclusions We demonstrated a characteristic colonic miR pattern in pIBD that could facilitate deeper understanding of the pathomechanism of IBD and may serve as a diagnostic tool.

Original languageEnglish
Pages (from-to)378-387
Number of pages10
JournalDigestive and Liver Disease
Volume49
Issue number4
DOIs
Publication statusPublished - Apr 1 2017

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Keywords

  • Inflammatory bowel disease
  • MicroRNA
  • Mucosal
  • Pediatric

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Cite this

Béres, N. J., Kiss, Z., Sztupinszki, Z., Lendvai, G., Arató, A., Sziksz, E., Vannay, Á., Szabó, A. J., Müller, K. E., Cseh, Á., Boros, K., & Verès, G. (2017). Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease. Digestive and Liver Disease, 49(4), 378-387. https://doi.org/10.1016/j.dld.2016.12.022