Altered mitochondrial response to activation of T-cells in neonate

G. Mészáros, Cs Orbán, A. Kaposi, G. Toldi, B. Gyarmati, T. Tulassay, B. Vásárhelyi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mitochondrial functions have a major impact on T-cell functionality. In this study we characterized whether mitochondrial function in the neonatal T-cells differs from that in the adult T-cells during short T-cell activation. Methods: We used flow cytometry methods to test mitochondrial mass and to monitor mitochondrial Ca2+levels, mitochondrial potential and superoxide generation in parallel with cytoplasmic Ca2+levels during phythohaemagglutinine-induced activation of CD4+ and CD8+ T-cells of 12 term neonates and 11 healthy adults. Results: Baseline mitochondrial mass of CD4+ and CD8+ cells was lower in the neonate than in the adult. In comparison with the adult, neonatal resting CD4+ T-cells had lower cytoplasmic Ca2+levels and this was associated with normal activation induced Ca2+-response. During short-term activation cytoplasmic Ca2+-response was lower in neonatal than in adult CD8+ T-cells. Mitochondrial Ca2+uptake was increased in CD4+ neonatal T cells while it decreased in CD8+ T-cells. Mitochondrial depolarization was increased in CD4+ and decreased in CD8+ neonatal T-cells compared to adults. Superoxide generation was higher and equal in neonatal CD4+ and CD8+ cells, respectively, compared to the adult ones. Conclusion: Our data suggest that neonatal T-cells exhibit marked differences in mitochondrial function and superoxide generation compared to adult T-cells.

Original languageEnglish
Pages (from-to)216-227
Number of pages12
JournalActa Physiologica Hungarica
Volume102
Issue number2
DOIs
Publication statusPublished - Jun 1 2015

Fingerprint

Newborn Infant
T-Lymphocytes
Superoxides
Flow Cytometry

Keywords

  • Calcium
  • Flow cytometry
  • Mitochondrial membrane potential
  • Neonate
  • Superoxide
  • T-cell

ASJC Scopus subject areas

  • Physiology (medical)
  • Medicine(all)

Cite this

Altered mitochondrial response to activation of T-cells in neonate. / Mészáros, G.; Orbán, Cs; Kaposi, A.; Toldi, G.; Gyarmati, B.; Tulassay, T.; Vásárhelyi, B.

In: Acta Physiologica Hungarica, Vol. 102, No. 2, 01.06.2015, p. 216-227.

Research output: Contribution to journalArticle

Mészáros, G. ; Orbán, Cs ; Kaposi, A. ; Toldi, G. ; Gyarmati, B. ; Tulassay, T. ; Vásárhelyi, B. / Altered mitochondrial response to activation of T-cells in neonate. In: Acta Physiologica Hungarica. 2015 ; Vol. 102, No. 2. pp. 216-227.
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