Altered mitochondrial response to activation of T-cells in neonate

G. Mészáros, Cs Orbán, A. Kaposi, G. Toldi, B. Gyarmati, T. Tulassay, B. Vásárhelyi

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4 Citations (Scopus)

Abstract

Mitochondrial functions have a major impact on T-cell functionality. In this study we characterized whether mitochondrial function in the neonatal T-cells differs from that in the adult T-cells during short T-cell activation. Methods: We used flow cytometry methods to test mitochondrial mass and to monitor mitochondrial Ca2+levels, mitochondrial potential and superoxide generation in parallel with cytoplasmic Ca2+levels during phythohaemagglutinine-induced activation of CD4+ and CD8+ T-cells of 12 term neonates and 11 healthy adults. Results: Baseline mitochondrial mass of CD4+ and CD8+ cells was lower in the neonate than in the adult. In comparison with the adult, neonatal resting CD4+ T-cells had lower cytoplasmic Ca2+levels and this was associated with normal activation induced Ca2+-response. During short-term activation cytoplasmic Ca2+-response was lower in neonatal than in adult CD8+ T-cells. Mitochondrial Ca2+uptake was increased in CD4+ neonatal T cells while it decreased in CD8+ T-cells. Mitochondrial depolarization was increased in CD4+ and decreased in CD8+ neonatal T-cells compared to adults. Superoxide generation was higher and equal in neonatal CD4+ and CD8+ cells, respectively, compared to the adult ones. Conclusion: Our data suggest that neonatal T-cells exhibit marked differences in mitochondrial function and superoxide generation compared to adult T-cells.

Original languageEnglish
Pages (from-to)216-227
Number of pages12
JournalActa physiologica Hungarica
Volume102
Issue number2
DOIs
Publication statusPublished - Jun 2015

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Keywords

  • Calcium
  • Flow cytometry
  • Mitochondrial membrane potential
  • Neonate
  • Superoxide
  • T-cell

ASJC Scopus subject areas

  • Physiology (medical)

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