Altered levels of mRNA expression and pharmacological reactivity of α1-adrenergic receptor subtypes in the late-pregnant rat myometrium

Eszter Ducza, R. Gáspár, G. Falkay

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14 Citations (Scopus)

Abstract

The adrenergic system plays a major role in the regulation of the uterine contractility during pregnancy. Our previous studies have shown the significance of the α1-adrenergic receptors (ARs) in the control of pregnant uterine contractility both in vitro and in vivo. Our present aim was to determine the changes in mRNA expression and pharmacological reactivity of the α1-ARs on days 18, 20, and 22 of pregnancy. To demonstrate the expressions of α1-AR subtype mRNA, we used a reverse transcription-polymerase chain reaction (RT-PCR); the pharmacological reactivity was tested by electric field stimulation (EFS). The expression of α1A-AR mRNA increased from day 18 to 22, while no α1B-AR mRNA was detectable. We found a small increase in the expression of α1D-AR mRNA on day 20, which was not followed by a significant change in pharmacological reactivity. The α1D-receptor expression and pharmacological reactivity decreased significantly up to day 22. EFS studies revealed that the α1A-AR antagonist 5-methylurapidil had EC50 values (1.9 × 10-6 -6.3 × 10-6 M) about one order of magnitude lower than those of the α1D-AR antagonist BMY7378(4 × 10-6 -3.6 × 10-5M). However, the α1B-AR antagonist cyclazosine exerted only a slight effect on the stimulated contractions. Strong correlations were found between the α1A-mRNA expression and the EC50 of 5-methylurapidil (r2 = 0.9712), and between the α1D-AR mRNA expression and the EC50 of BMY 7378 (r2 = 0.9937). Our findings suggest that both α1A- and α1D-ARs are involved in the regulation of the pregnant uterine contractility. The density and pharmacological reactivity indicate that the α1A-AR seems to play the major role in late-pregnant myometrial contraction.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalMolecular Reproduction and Development
Volume62
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Myometrium
Adrenergic Receptors
Pharmacology
Messenger RNA
Adrenergic Antagonists
Electric Stimulation
Uterine Contraction
Pregnancy
Adrenergic Agents
Reverse Transcription
Polymerase Chain Reaction

Keywords

  • α1-adrenergic receptors subtypes
  • Electric field stimulation
  • Late-pregnant rat
  • Pharmacological reactivity
  • RT-PCR

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

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abstract = "The adrenergic system plays a major role in the regulation of the uterine contractility during pregnancy. Our previous studies have shown the significance of the α1-adrenergic receptors (ARs) in the control of pregnant uterine contractility both in vitro and in vivo. Our present aim was to determine the changes in mRNA expression and pharmacological reactivity of the α1-ARs on days 18, 20, and 22 of pregnancy. To demonstrate the expressions of α1-AR subtype mRNA, we used a reverse transcription-polymerase chain reaction (RT-PCR); the pharmacological reactivity was tested by electric field stimulation (EFS). The expression of α1A-AR mRNA increased from day 18 to 22, while no α1B-AR mRNA was detectable. We found a small increase in the expression of α1D-AR mRNA on day 20, which was not followed by a significant change in pharmacological reactivity. The α1D-receptor expression and pharmacological reactivity decreased significantly up to day 22. EFS studies revealed that the α1A-AR antagonist 5-methylurapidil had EC50 values (1.9 × 10-6 -6.3 × 10-6 M) about one order of magnitude lower than those of the α1D-AR antagonist BMY7378(4 × 10-6 -3.6 × 10-5M). However, the α1B-AR antagonist cyclazosine exerted only a slight effect on the stimulated contractions. Strong correlations were found between the α1A-mRNA expression and the EC50 of 5-methylurapidil (r2 = 0.9712), and between the α1D-AR mRNA expression and the EC50 of BMY 7378 (r2 = 0.9937). Our findings suggest that both α1A- and α1D-ARs are involved in the regulation of the pregnant uterine contractility. The density and pharmacological reactivity indicate that the α1A-AR seems to play the major role in late-pregnant myometrial contraction.",
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AU - Gáspár, R.

AU - Falkay, G.

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N2 - The adrenergic system plays a major role in the regulation of the uterine contractility during pregnancy. Our previous studies have shown the significance of the α1-adrenergic receptors (ARs) in the control of pregnant uterine contractility both in vitro and in vivo. Our present aim was to determine the changes in mRNA expression and pharmacological reactivity of the α1-ARs on days 18, 20, and 22 of pregnancy. To demonstrate the expressions of α1-AR subtype mRNA, we used a reverse transcription-polymerase chain reaction (RT-PCR); the pharmacological reactivity was tested by electric field stimulation (EFS). The expression of α1A-AR mRNA increased from day 18 to 22, while no α1B-AR mRNA was detectable. We found a small increase in the expression of α1D-AR mRNA on day 20, which was not followed by a significant change in pharmacological reactivity. The α1D-receptor expression and pharmacological reactivity decreased significantly up to day 22. EFS studies revealed that the α1A-AR antagonist 5-methylurapidil had EC50 values (1.9 × 10-6 -6.3 × 10-6 M) about one order of magnitude lower than those of the α1D-AR antagonist BMY7378(4 × 10-6 -3.6 × 10-5M). However, the α1B-AR antagonist cyclazosine exerted only a slight effect on the stimulated contractions. Strong correlations were found between the α1A-mRNA expression and the EC50 of 5-methylurapidil (r2 = 0.9712), and between the α1D-AR mRNA expression and the EC50 of BMY 7378 (r2 = 0.9937). Our findings suggest that both α1A- and α1D-ARs are involved in the regulation of the pregnant uterine contractility. The density and pharmacological reactivity indicate that the α1A-AR seems to play the major role in late-pregnant myometrial contraction.

AB - The adrenergic system plays a major role in the regulation of the uterine contractility during pregnancy. Our previous studies have shown the significance of the α1-adrenergic receptors (ARs) in the control of pregnant uterine contractility both in vitro and in vivo. Our present aim was to determine the changes in mRNA expression and pharmacological reactivity of the α1-ARs on days 18, 20, and 22 of pregnancy. To demonstrate the expressions of α1-AR subtype mRNA, we used a reverse transcription-polymerase chain reaction (RT-PCR); the pharmacological reactivity was tested by electric field stimulation (EFS). The expression of α1A-AR mRNA increased from day 18 to 22, while no α1B-AR mRNA was detectable. We found a small increase in the expression of α1D-AR mRNA on day 20, which was not followed by a significant change in pharmacological reactivity. The α1D-receptor expression and pharmacological reactivity decreased significantly up to day 22. EFS studies revealed that the α1A-AR antagonist 5-methylurapidil had EC50 values (1.9 × 10-6 -6.3 × 10-6 M) about one order of magnitude lower than those of the α1D-AR antagonist BMY7378(4 × 10-6 -3.6 × 10-5M). However, the α1B-AR antagonist cyclazosine exerted only a slight effect on the stimulated contractions. Strong correlations were found between the α1A-mRNA expression and the EC50 of 5-methylurapidil (r2 = 0.9712), and between the α1D-AR mRNA expression and the EC50 of BMY 7378 (r2 = 0.9937). Our findings suggest that both α1A- and α1D-ARs are involved in the regulation of the pregnant uterine contractility. The density and pharmacological reactivity indicate that the α1A-AR seems to play the major role in late-pregnant myometrial contraction.

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