Altered inactivation of trinitrophenylated thrombin by antithrombin iii in the presence of heparin

R. MacHovich, E. Regoeczi, M. W.C. Hatton

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Abstract

Modification of bovine α-thrombin by exposure to 2,4,6-trinitrobenzenesulphonic acid and the effects of the modification on the inactivation of the enzyme in the presence of heparin are reported. Transformation of four-five lysine residues resulted in a decrease of the clotting activity of the enzyme by 30-40%, yet its interaction with antithrombin III was not significantly impaired. However, the catalytic effect of heparin on the thrombin-antithrombin III reaction was grossly reduced and the potentiating effects of NaCl and CaC12 on heparin-mediated catalysis was either minimized or no longer apparent.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalThrombosis research
Volume17
Issue number3-4
DOIs
Publication statusPublished - Jan 1 1980

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ASJC Scopus subject areas

  • Hematology

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