Altered gene expression profiles in the hippocampus and prefrontal cortex of type 2 diabetic rats

Omar Abdul-Rahman, M. Sasvári, A. Vér, Klara Rosta, Bernadett K. Szasz, Eva Kereszturi, Gergely Keszler

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background: There has been an increasing body of epidemiologic and biochemical evidence implying the role of cerebral insulin resistance in Alzheimer-type dementia. For a better understanding of the insulin effect on the central nervous system, we performed microarray-based global gene expression profiling in the hippocampus, striatum and prefrontal cortex of streptozotocin-induced and spontaneously diabetic Goto-Kakizaki rats as model animals for type 1 and type 2 diabetes, respectively.Results: Following pathway analysis and validation of gene lists by real-time polymerase chain reaction, 30 genes from the hippocampus, such as the inhibitory neuropeptide galanin, synuclein gamma and uncoupling protein 2, and 22 genes from the prefrontal cortex, e.g. galanin receptor 2, protein kinase C gamma and epsilon, ABCA1 (ATP-Binding Cassette A1), CD47 (Cluster of Differentiation 47) and the RET (Rearranged During Transfection) protooncogene, were found to exhibit altered expression levels in type 2 diabetic model animals in comparison to non-diabetic control animals. These gene lists proved to be partly overlapping and encompassed genes related to neurotransmission, lipid metabolism, neuronal development, insulin secretion, oxidative damage and DNA repair. On the other hand, no significant alterations were found in the transcriptomes of the corpus striatum in the same animals. Changes in the cerebral gene expression profiles seemed to be specific for the type 2 diabetic model, as no such alterations were found in streptozotocin-treated animals.Conclusions: According to our knowledge this is the first characterization of the whole-genome expression changes of specific brain regions in a diabetic model. Our findings shed light on the complex role of insulin signaling in fine-tuning brain functions, and provide further experimental evidence in support of the recently elaborated theory of type 3 diabetes.

Original languageEnglish
Article number81
JournalBMC Genomics
Volume13
Issue number1
DOIs
Publication statusPublished - Feb 27 2012

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Prefrontal Cortex
Transcriptome
Hippocampus
Insulin
Streptozocin
Genes
Receptor, Galanin, Type 2
Ethenoadenosine Triphosphate
Animal Models
Synucleins
Overlapping Genes
Protein Kinase C-epsilon
Galanin
Corpus Striatum
Brain
Gene Expression Profiling
Neuropeptides
Type 1 Diabetes Mellitus
Lipid Metabolism
Synaptic Transmission

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

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Altered gene expression profiles in the hippocampus and prefrontal cortex of type 2 diabetic rats. / Abdul-Rahman, Omar; Sasvári, M.; Vér, A.; Rosta, Klara; Szasz, Bernadett K.; Kereszturi, Eva; Keszler, Gergely.

In: BMC Genomics, Vol. 13, No. 1, 81, 27.02.2012.

Research output: Contribution to journalArticle

Abdul-Rahman, Omar ; Sasvári, M. ; Vér, A. ; Rosta, Klara ; Szasz, Bernadett K. ; Kereszturi, Eva ; Keszler, Gergely. / Altered gene expression profiles in the hippocampus and prefrontal cortex of type 2 diabetic rats. In: BMC Genomics. 2012 ; Vol. 13, No. 1.
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