Altered balance between extracellular proteolysis and antiproteolysis is associated with adaptive coronary arteriogenesis

Wei jun Cai, Regina Vosschulte, Abdollah Afsah-Hedjri, Sophie Koltai, E. Kocsis, Dimitri Scholz, Sawa Kostin, Wolfgang Schaper, Jutta Schaper

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Abstract

To study the role of extracellular proteolysis and antiproteolysis during adaptive arteriogenesis (collateral vessel growth) we took 58 collaterals at various developmental stages from 14 dogs with chronic occlusion of the left circumflex coronary artery (LCx) by ameroid constrictor. Immunofluorence and quantitative immunofluorescence with antibodies against α-smooth muscle actin, desmin, matrix metalloproteinases 2 (MMP-2), MMP-9. tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP-2). urokinase-type plasminogen activator (u-PA) and its inhibitor-1 (PAI-1) were studied with confocal microscopy. Additionally, SDS-PAGE zymography was employed, We found that in normal coronary arteries. MMP-2. MMP-9 and PAI-1 were present in all layers of the wall in small amounts. TIMP-1 was found only in smooth muscle cells. In contrast, in growing collaterals, MMP-2 and MMP-9 were 3.4-fold and 4.1-fold higher in the neointima than in the media respectively. TIMP-1 was 4.4-fold higher in the media over the growing neointima. Zymography showed MMP-2 and MMP-9 activated. PAI-1 was increased, especially in the growing neointima where it was 1.4-fold higher. In mature collaterals. MMP-2 and MMP-9 were downregulated in the neointima, 1.4-fold and 1.3-fold higher over the media. TIMP-1 was 1.4-fold increased in the neointima but PAI-1 was downregulated. Desmin and α-smooth muscle actin were significantly increased in the neointima compared to growing vessels. U-PA was moderately increased in growing vessels. TIMP-2 was not detectable in collaterals. We conclude that expression of MMP-2 and 9. TIMP-1 and PAI-1 showed a spatial and temporal pattern which is closely associated with the development of collateral vessels. The shift of the balance between proteolysis and antiproteolysis is regulated not only by MMPs and TIMP-1, but also by the PA-PAI system. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)997-1011
Number of pages15
JournalJournal of Molecular and Cellular Cardiology
Volume32
Issue number6
DOIs
Publication statusPublished - 2000

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Tissue Inhibitor of Metalloproteinase-1
Neointima
Matrix Metalloproteinase 2
Plasminogen Activator Inhibitor 1
Proteolysis
Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Desmin
Urokinase-Type Plasminogen Activator
Smooth Muscle
Actins
Coronary Vessels
Down-Regulation
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 9
Confocal Microscopy
Smooth Muscle Myocytes
Fluorescent Antibody Technique
Polyacrylamide Gel Electrophoresis
Dogs

Keywords

  • Arteriogenesis
  • Collateral vessel growth
  • Dog
  • Extracellular matrix
  • Metalloproteinases
  • Vascular remodeling

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Altered balance between extracellular proteolysis and antiproteolysis is associated with adaptive coronary arteriogenesis. / Cai, Wei jun; Vosschulte, Regina; Afsah-Hedjri, Abdollah; Koltai, Sophie; Kocsis, E.; Scholz, Dimitri; Kostin, Sawa; Schaper, Wolfgang; Schaper, Jutta.

In: Journal of Molecular and Cellular Cardiology, Vol. 32, No. 6, 2000, p. 997-1011.

Research output: Contribution to journalArticle

Cai, Wei jun ; Vosschulte, Regina ; Afsah-Hedjri, Abdollah ; Koltai, Sophie ; Kocsis, E. ; Scholz, Dimitri ; Kostin, Sawa ; Schaper, Wolfgang ; Schaper, Jutta. / Altered balance between extracellular proteolysis and antiproteolysis is associated with adaptive coronary arteriogenesis. In: Journal of Molecular and Cellular Cardiology. 2000 ; Vol. 32, No. 6. pp. 997-1011.
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AU - Vosschulte, Regina

AU - Afsah-Hedjri, Abdollah

AU - Koltai, Sophie

AU - Kocsis, E.

AU - Scholz, Dimitri

AU - Kostin, Sawa

AU - Schaper, Wolfgang

AU - Schaper, Jutta

PY - 2000

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N2 - To study the role of extracellular proteolysis and antiproteolysis during adaptive arteriogenesis (collateral vessel growth) we took 58 collaterals at various developmental stages from 14 dogs with chronic occlusion of the left circumflex coronary artery (LCx) by ameroid constrictor. Immunofluorence and quantitative immunofluorescence with antibodies against α-smooth muscle actin, desmin, matrix metalloproteinases 2 (MMP-2), MMP-9. tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP-2). urokinase-type plasminogen activator (u-PA) and its inhibitor-1 (PAI-1) were studied with confocal microscopy. Additionally, SDS-PAGE zymography was employed, We found that in normal coronary arteries. MMP-2. MMP-9 and PAI-1 were present in all layers of the wall in small amounts. TIMP-1 was found only in smooth muscle cells. In contrast, in growing collaterals, MMP-2 and MMP-9 were 3.4-fold and 4.1-fold higher in the neointima than in the media respectively. TIMP-1 was 4.4-fold higher in the media over the growing neointima. Zymography showed MMP-2 and MMP-9 activated. PAI-1 was increased, especially in the growing neointima where it was 1.4-fold higher. In mature collaterals. MMP-2 and MMP-9 were downregulated in the neointima, 1.4-fold and 1.3-fold higher over the media. TIMP-1 was 1.4-fold increased in the neointima but PAI-1 was downregulated. Desmin and α-smooth muscle actin were significantly increased in the neointima compared to growing vessels. U-PA was moderately increased in growing vessels. TIMP-2 was not detectable in collaterals. We conclude that expression of MMP-2 and 9. TIMP-1 and PAI-1 showed a spatial and temporal pattern which is closely associated with the development of collateral vessels. The shift of the balance between proteolysis and antiproteolysis is regulated not only by MMPs and TIMP-1, but also by the PA-PAI system. (C) 2000 Academic Press.

AB - To study the role of extracellular proteolysis and antiproteolysis during adaptive arteriogenesis (collateral vessel growth) we took 58 collaterals at various developmental stages from 14 dogs with chronic occlusion of the left circumflex coronary artery (LCx) by ameroid constrictor. Immunofluorence and quantitative immunofluorescence with antibodies against α-smooth muscle actin, desmin, matrix metalloproteinases 2 (MMP-2), MMP-9. tissue inhibitor of metalloproteinases 1 (TIMP-1) and 2 (TIMP-2). urokinase-type plasminogen activator (u-PA) and its inhibitor-1 (PAI-1) were studied with confocal microscopy. Additionally, SDS-PAGE zymography was employed, We found that in normal coronary arteries. MMP-2. MMP-9 and PAI-1 were present in all layers of the wall in small amounts. TIMP-1 was found only in smooth muscle cells. In contrast, in growing collaterals, MMP-2 and MMP-9 were 3.4-fold and 4.1-fold higher in the neointima than in the media respectively. TIMP-1 was 4.4-fold higher in the media over the growing neointima. Zymography showed MMP-2 and MMP-9 activated. PAI-1 was increased, especially in the growing neointima where it was 1.4-fold higher. In mature collaterals. MMP-2 and MMP-9 were downregulated in the neointima, 1.4-fold and 1.3-fold higher over the media. TIMP-1 was 1.4-fold increased in the neointima but PAI-1 was downregulated. Desmin and α-smooth muscle actin were significantly increased in the neointima compared to growing vessels. U-PA was moderately increased in growing vessels. TIMP-2 was not detectable in collaterals. We conclude that expression of MMP-2 and 9. TIMP-1 and PAI-1 showed a spatial and temporal pattern which is closely associated with the development of collateral vessels. The shift of the balance between proteolysis and antiproteolysis is regulated not only by MMPs and TIMP-1, but also by the PA-PAI system. (C) 2000 Academic Press.

KW - Arteriogenesis

KW - Collateral vessel growth

KW - Dog

KW - Extracellular matrix

KW - Metalloproteinases

KW - Vascular remodeling

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