Altered activation of peripheral CD8+ T cells in pediatric Crohn's disease

Csaba Orbán, Dolóresz Szabó, Anna Bajnok, B. Vásárhelyi, T. Tulassay, A. Arató, G. Veres, Gergely Toldi

Research output: Contribution to journalArticle

Abstract

Aim Although Crohn's disease (CD) is an extensively investigated autoimmune condition, knowledge on early phase activation of lymphocytes, especially CD8+ Tc cells is scarce. Our aim was to investigate the calcium influx characteristics of CD8+ cells upon activation as well as the expression and function of Kv1.3 and IKCa1 lymphocyte potassium channels. Methods We took peripheral blood from 12 healthy controls, 23 CD children on conventional therapy and 6 severe CD children before and after infliximab therapy. Intracellular calcium levels were monitored in CD8+ lymphocytes using flow cytometry. Results In CD treated with standard therapy calcium response during activation was elevated. This was not affected by the inhibition of Kv1.3 or IKCa1 potassium channels. After the switch to infliximab potassium channel function and expression of CD8+ lymphocytes were comparable to healthy controls in severe CD. Conclusion Calcium handling of CD8+ lymphocytes is altered in pediatric CD, which is normalized by infliximab therapy.

Original languageEnglish
Pages (from-to)48-51
Number of pages4
JournalImmunology Letters
Volume185
DOIs
Publication statusPublished - May 1 2017

    Fingerprint

Keywords

  • Calcium influx
  • CD8
  • Crohn's disease
  • Infliximab
  • T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this