Alterations of NADPH

protochlorophyllide oxidoreductase quantity and lipid composition in etiolated barley seedlings infected by Barley stripe mosaic virus (BSMV)

Anett Harsányi, Margareta Ryberg, Mats X. Andersson, K. Bóka, L. László, Gergely Botond, B. Böddi, Richard Gáborjányi

Research output: Contribution to journalArticle

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Abstract

To understand the phenomenon by which infection of seed-transmitted Barley stripe mosaic virus (BSMV) alters membrane structures and inhibits protochlorophyllide biosynthesis of dark-grown barley (Hordeum vulgare L.) plants, we analysed the presence of NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) and the galactolipid content and fatty acid composition. The amount of POR in etioplasts of infected leaves, compared with non-infected leaves, was reduced, as measured by immunoelectron microscopy and Western blot. These results are in agreement with the previously described reduction of the ratio of the photoactive 650 nm to non-photoactive 630 nm absorbing protochlorophyllide forms (Harsányi et al., 2002. Physiol. Plant 114, 149-155). The galactolipid content was lower in infected leaves. Monogalactosyl-diacylglycerol (MGDG) content was reduced to 40% and digalactosyl-diacylglycerol to 55% of control plants on a fresh weight basis. In infected plants, the proportion of linolenic acid decreased in both galactolipids. The lower amount of highly unsaturated fatty acids and the reduced abundance of MGDG correlated well with the previously detected reduction in the membrane ratio of prolamellar body (PLB) to prothylakoid (Harsányi et al., 2002. Physiol. Plant 114, 149-155). The reduced amount of POR and the above described alterations in the lipid composition resulted in a disturbed structure of PLBs. As a consequence, pigment synthesis and the greening process were inhibited in infected cells, in turn explaining the appearance of chlorotic stripes of BSMV-infected barley leaves. Our results show that BSMV infection can be detected at a very early stage of leaf development.

Original languageEnglish
Pages (from-to)533-541
Number of pages9
JournalMolecular Plant Pathology
Volume7
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

protochlorophyllide reductase
Barley stripe mosaic virus
protochlorophyllides
Mosaic Viruses
oxidoreductases
Hordeum
lipid composition
Seedlings
NADP (coenzyme)
barley
virus
galactolipids
Galactolipids
lipid
seedling
Lipids
diacylglycerols
seedlings
Diglycerides
Protochlorophyllide

ASJC Scopus subject areas

  • Plant Science

Cite this

Alterations of NADPH : protochlorophyllide oxidoreductase quantity and lipid composition in etiolated barley seedlings infected by Barley stripe mosaic virus (BSMV). / Harsányi, Anett; Ryberg, Margareta; Andersson, Mats X.; Bóka, K.; László, L.; Botond, Gergely; Böddi, B.; Gáborjányi, Richard.

In: Molecular Plant Pathology, Vol. 7, No. 6, 2006, p. 533-541.

Research output: Contribution to journalArticle

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abstract = "To understand the phenomenon by which infection of seed-transmitted Barley stripe mosaic virus (BSMV) alters membrane structures and inhibits protochlorophyllide biosynthesis of dark-grown barley (Hordeum vulgare L.) plants, we analysed the presence of NADPH:protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) and the galactolipid content and fatty acid composition. The amount of POR in etioplasts of infected leaves, compared with non-infected leaves, was reduced, as measured by immunoelectron microscopy and Western blot. These results are in agreement with the previously described reduction of the ratio of the photoactive 650 nm to non-photoactive 630 nm absorbing protochlorophyllide forms (Hars{\'a}nyi et al., 2002. Physiol. Plant 114, 149-155). The galactolipid content was lower in infected leaves. Monogalactosyl-diacylglycerol (MGDG) content was reduced to 40{\%} and digalactosyl-diacylglycerol to 55{\%} of control plants on a fresh weight basis. In infected plants, the proportion of linolenic acid decreased in both galactolipids. The lower amount of highly unsaturated fatty acids and the reduced abundance of MGDG correlated well with the previously detected reduction in the membrane ratio of prolamellar body (PLB) to prothylakoid (Hars{\'a}nyi et al., 2002. Physiol. Plant 114, 149-155). The reduced amount of POR and the above described alterations in the lipid composition resulted in a disturbed structure of PLBs. As a consequence, pigment synthesis and the greening process were inhibited in infected cells, in turn explaining the appearance of chlorotic stripes of BSMV-infected barley leaves. Our results show that BSMV infection can be detected at a very early stage of leaf development.",
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AU - Bóka, K.

AU - László, L.

AU - Botond, Gergely

AU - Böddi, B.

AU - Gáborjányi, Richard

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