Alterations in Protein Synthesis in the Cyanobacterium Synechococcus sp. Strain PCC-6301 in Response to Calendula micrantha Extract with Molluscicidal Activity

O. Hammouda, G. Borbély

Research output: Contribution to journalArticle

Abstract

The response to the extract of the Egyptian wild herb Calendula micrantha, with molluscicidal activity,was examined in the unicellular cyanobacterium Synechococcus sp. strain PCC 6301. Growth and chlorophyll a of the cells were only slightly affected by low concentrations but drastically reduced by high concentrations. The rate of protein synthesis progressively decreased by increasing extract concentration. The cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161K (161,000), 96.7K, 93.4K, 85K, 69.9K, 59K, 49K, 45K, 35K, 32.4K, 28K, 24K, 21.7K, 18K, and 16K based on their mobilities in gel electrophoresis. These initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. Some of the induced polypeptides are similar to that known to occur in other stresses, especially heat-shock stress.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalEcotoxicology and Environmental Safety
Volume31
Issue number3
DOIs
Publication statusPublished - Aug 1995

Fingerprint

Calendula
Synechococcus
Polypeptides
Cyanobacteria
cyanobacterium
Proteins
Peptides
protein
Plant extracts
plant extract
heat shock
Plant Extracts
Chlorophyll
Electrophoresis
herb
Shock
electrokinesis
chlorophyll a
Gels
gel

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Pollution
  • Health, Toxicology and Mutagenesis
  • Environmental Chemistry
  • Environmental Science(all)

Cite this

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abstract = "The response to the extract of the Egyptian wild herb Calendula micrantha, with molluscicidal activity,was examined in the unicellular cyanobacterium Synechococcus sp. strain PCC 6301. Growth and chlorophyll a of the cells were only slightly affected by low concentrations but drastically reduced by high concentrations. The rate of protein synthesis progressively decreased by increasing extract concentration. The cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161K (161,000), 96.7K, 93.4K, 85K, 69.9K, 59K, 49K, 45K, 35K, 32.4K, 28K, 24K, 21.7K, 18K, and 16K based on their mobilities in gel electrophoresis. These initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. Some of the induced polypeptides are similar to that known to occur in other stresses, especially heat-shock stress.",
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N2 - The response to the extract of the Egyptian wild herb Calendula micrantha, with molluscicidal activity,was examined in the unicellular cyanobacterium Synechococcus sp. strain PCC 6301. Growth and chlorophyll a of the cells were only slightly affected by low concentrations but drastically reduced by high concentrations. The rate of protein synthesis progressively decreased by increasing extract concentration. The cells preferentially induced the synthesis of a limited number of polypeptides in response to the treatment. Among the induced polypeptides were those with apparent molecular weights of 161K (161,000), 96.7K, 93.4K, 85K, 69.9K, 59K, 49K, 45K, 35K, 32.4K, 28K, 24K, 21.7K, 18K, and 16K based on their mobilities in gel electrophoresis. These initial studies suggest that the plant extract exerted certain stress which stimulated alteration in the pattern of protein synthesis in Synechococcus sp. Some of the induced polypeptides are similar to that known to occur in other stresses, especially heat-shock stress.

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