Alteration of the N-glycome of bovine milk glycoproteins during early lactation

Shota Takimori, Hideyuki Shimaoka, Jun Ichi Furukawa, Tadashi Yamashita, Maho Amano, Naoki Fujitani, Yasuhiro Takegawa, Lennart Hammarström, I. Kacskovics, Yasuro Shinohara, Shin Ichiro Nishimura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Milk provides nutritional, immunological and developmental components for newborns. Whereas identification of such components has been performed by targeting proteins and free oligosaccharides, structural and functional analyses of the N-glycome of milk glycoproteins are scarce. In this study, we investigated, for the first time, the alterations of the bovine milk N-glycome during early lactation (1 day, 1, 2, 3 and 4 weeks postpartum), characterizing more than 80 N-glycans. The glycomic profile of colostrum on day 1 after calving differed substantially from that in other periods during early lactation. The proteins in colostrum obtained 1 day postpartum were more highly sialylated than milk samples obtained at other time points, and the N-glycolylneuraminic acid (Neu5Gc)/N-acetylneuraminic acid (Neu5Ac) ratio was significantly higher on day 1, showing a gradual decline with time. In order to dissect the N-glycome of colostrum, alterations of the N-glycosylation profile of major bovine milk proteins during the early lactation stage were elucidated, revealing that the alteration is largely attributable to qualitative and quantitative N-glycosylation changes of IgG, the major glycoprotein in colostrum. Furthermore, by preparing and analyzing IgGs in which the N-glycan structure and subtypes were well characterized, we found that the interaction between IgG and FcRn was not affected by the structure of the N-glycans attached to IgG. We also found that bovine FcRn binds IgG2 better than IgG1, strongly suggesting that the role of FcRn in the bovine mammary gland is to recycle IgG2 from the udder to blood, rather than to secrete IgG 1 into colostrum. Unique alteration of bovine milk N-glycome during early lactation revealed by glycoblotting and MALDI-TOF MS analyses was largely attributable to the dramatic change of the IgG glycosylation pattern around parturition. Bovine FcRn binds IgG2 better than IgG1 suggesting the role of FcRn in the bovine mammary gland is to recycle IgG2 from the udder to blood.

Original languageEnglish
Pages (from-to)3769-3781
Number of pages13
JournalFEBS Journal
Volume278
Issue number19
DOIs
Publication statusPublished - Oct 2011

Fingerprint

Lactation
Glycoproteins
Milk
Immunoglobulin G
Colostrum
Glycosylation
Polysaccharides
Animal Mammary Glands
Human Mammary Glands
Postpartum Period
Blood
Glycomics
Milk Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
N-Acetylneuraminic Acid
Protein Transport
Oligosaccharides
Proteins
Parturition

Keywords

  • bovine milk glycoproteins
  • colostrum
  • FcRn
  • glycomics
  • IgG

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Takimori, S., Shimaoka, H., Furukawa, J. I., Yamashita, T., Amano, M., Fujitani, N., ... Nishimura, S. I. (2011). Alteration of the N-glycome of bovine milk glycoproteins during early lactation. FEBS Journal, 278(19), 3769-3781. https://doi.org/10.1111/j.1742-4658.2011.08299.x

Alteration of the N-glycome of bovine milk glycoproteins during early lactation. / Takimori, Shota; Shimaoka, Hideyuki; Furukawa, Jun Ichi; Yamashita, Tadashi; Amano, Maho; Fujitani, Naoki; Takegawa, Yasuhiro; Hammarström, Lennart; Kacskovics, I.; Shinohara, Yasuro; Nishimura, Shin Ichiro.

In: FEBS Journal, Vol. 278, No. 19, 10.2011, p. 3769-3781.

Research output: Contribution to journalArticle

Takimori, S, Shimaoka, H, Furukawa, JI, Yamashita, T, Amano, M, Fujitani, N, Takegawa, Y, Hammarström, L, Kacskovics, I, Shinohara, Y & Nishimura, SI 2011, 'Alteration of the N-glycome of bovine milk glycoproteins during early lactation', FEBS Journal, vol. 278, no. 19, pp. 3769-3781. https://doi.org/10.1111/j.1742-4658.2011.08299.x
Takimori S, Shimaoka H, Furukawa JI, Yamashita T, Amano M, Fujitani N et al. Alteration of the N-glycome of bovine milk glycoproteins during early lactation. FEBS Journal. 2011 Oct;278(19):3769-3781. https://doi.org/10.1111/j.1742-4658.2011.08299.x
Takimori, Shota ; Shimaoka, Hideyuki ; Furukawa, Jun Ichi ; Yamashita, Tadashi ; Amano, Maho ; Fujitani, Naoki ; Takegawa, Yasuhiro ; Hammarström, Lennart ; Kacskovics, I. ; Shinohara, Yasuro ; Nishimura, Shin Ichiro. / Alteration of the N-glycome of bovine milk glycoproteins during early lactation. In: FEBS Journal. 2011 ; Vol. 278, No. 19. pp. 3769-3781.
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