Chitin synthase activity in total membrane fractions from two polyene-resistant, ergosterol-deficient mutants of Candida albicans was significantly higher in comparison to the parental polyene-sensitive strain. The zymogen component from membrane preparations of stationaryphase cells of ergosterol mutants was more susceptible to trypsin digestion than those from the parental polyene-sensitive strain. Mechanisms that might explain the effect of changes in membrane composition in the mutant strains on chitin synthase activity are discussed.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology