Alpha-adrenergic receptor (α 2A) is colocalized in basal forebrain cholinergic neurons: A light and electron microscopic double immunolabeling study

L. Zaborszky, D. L. Rosin, J. Kiss

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Abstract

A variety of data suggest that noradrenaline and acetylcholine may interact in the basal forebrain, however no morphological studies have addressed whether indeed cholinergic neurons express adrenergic receptors. We have investigated the presence of alpha-adrenergic receptor subtype α 2A -AR in cholinergic neurons of the basal forebrain. Cholinergic neurons were identified with an antibody against choline acetyltransferase and the receptor with a polyclonal antibody raised against a 47 amino acid fragment of the third intracellular loop of the α 2A-AR. For double labeling at the light microscopic level the Ni-DAB/DAB technique was used, and for electron microscopy an immunoperoxidase/immunogold method was applied. We detected the α 2A-AR protein in cholinergic as well as in non-cholinergic neurons. Almost half of all cholinergic neurons contained this adrenergic receptor. Double-labeled neurons were distributed throughout the rostro-caudal extent of the basal forebrain cholinergic continuum, including the medial septum, vertical and horizontal diagonal band nuclei, pallidal regions, substantia innominata and the internal capsule. Non-cholinergic neurons that expressed the α 2A-AR outnumbered cholinergic/α 2A-AR neurons by several factors. Electron microscopy confirmed the presence of α 2A-AR in cholinergic neurons in the medial septum, vertical and horizontal diagonal band nuclei. Gold particles (10 nm) indicative of α 2A-AR were diffusely distributed in the cytoplasm and accumulated in cytoplasmic areas near the Golgi complex and cysterns of the endoplasmic reticulum and were associated with the cellular membranes at synaptic and non-synaptic locations. Since many of the α 2A-AR+/ non-cholinergic neurons we detected are likely to be GABAergic cells, our data support the hypothesis that noradrenaline may act via basal forebrain cholinergic and non-cholinergic neurons to influence cortical activity.

Original languageEnglish
Pages (from-to)265-276
Number of pages12
JournalJournal of Neurocytology
Volume33
Issue number3
DOIs
Publication statusPublished - May 2004

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Adrenergic alpha-2 Receptors
Cholinergic Neurons
Electrons
Neurons
Light
Cholinergic Agents
Septal Nuclei
Adrenergic Receptors
Norepinephrine
Electron Microscopy
Substantia Innominata
Internal Capsule
Synaptic Membranes
Choline O-Acetyltransferase
Antibodies
Golgi Apparatus
Gold
Endoplasmic Reticulum
Acetylcholine
Basal Forebrain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Anatomy
  • Cell Biology
  • Histology

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Alpha-adrenergic receptor (α 2A) is colocalized in basal forebrain cholinergic neurons : A light and electron microscopic double immunolabeling study. / Zaborszky, L.; Rosin, D. L.; Kiss, J.

In: Journal of Neurocytology, Vol. 33, No. 3, 05.2004, p. 265-276.

Research output: Contribution to journalArticle

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