Alpha-1 chain of human haptoglobin as viability marker of in vitro fertilized human embryos: information beyond morphology

Gergely Montskó, Krisztina Gödöny, Róbert Herczeg, Ákos Várnagy, József Bódis, G. Kovács

Research output: Contribution to journalArticle

Abstract

Only one third of the in vitro fertilization treatments result in successful delivery following morphological viability assessment worldwide. A paper by Montskó et al. (2015) describes the identification of the alpha-1 chain of human haptoglobin as a potential marker of embryo viability. Using mass spectrometry, the concentration of the haptoglobin alpha-1 chain was determined in spent culture media samples of in vitro fertilized embryos and correlation was found with the outcome of the respective transfer. In the present study we investigated, whether the concentration of haptoglobin alpha-1 chain shows any correlation with morphological scores to clarify whether levels of the alpha-1 chain provide additional information on embryo viability unnoticed by the morphological assessment. In the study, pregnancy and live birth rates were examined in 143 transferred samples of 86 patients, retrospectively. Two sample groups were created. The control group contained embryos classified as ‘good’ or ‘fair’ based on the Istanbul Consensus Criteria System, while the double-assay group contained embryos assessed as ‘good’ or ‘fair’ by the morphological evaluation and as ‘viable’ by the haptoglobin assay. Clinical pregnancy rate was 30.2% in the control group, while 47.6% in the group scored parallel with morphological criteria and proteomic analysis (p < 0.05). The increased clinical pregnancy rate observed in the double-assayed group can be attributed to decreased false-positivity of the double assay. Abbreviations: IVF: in vitro fertilization; SEC: spent embryo culture medium; HSA: human serum albumin; Hpt: haptoglobin; HptA1: haptoglobin alpha-1 chain; ICCS: Istanbul Consensus Criteria System; BMI: body mass index; ICSI: intracytoplasmic sperm injection.

Original languageEnglish
JournalSystems Biology in Reproductive Medicine
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Haptoglobins
Embryonic Structures
Pregnancy Rate
Intracytoplasmic Sperm Injections
Fertilization in Vitro
Culture Media
Control Groups
Serum Albumin
Proteomics
In Vitro Techniques
human HP protein
Mass Spectrometry
Body Mass Index

Keywords

  • biomarker
  • embryo viability
  • In vitro fertilization
  • mass spectrometry
  • success rate

ASJC Scopus subject areas

  • Reproductive Medicine
  • Urology

Cite this

Alpha-1 chain of human haptoglobin as viability marker of in vitro fertilized human embryos : information beyond morphology. / Montskó, Gergely; Gödöny, Krisztina; Herczeg, Róbert; Várnagy, Ákos; Bódis, József; Kovács, G.

In: Systems Biology in Reproductive Medicine, 01.01.2018.

Research output: Contribution to journalArticle

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