Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus

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Abstract

The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate. Copyright (C) 1998 Federation of European Microbiological Societies Published by Elsevier Scienced B.V. All rights reserved.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalFEMS Microbiology Letters
Volume164
Issue number2
DOIs
Publication statusPublished - Jul 15 1998

Fingerprint

allosamidin
Acremonium
Chitinases
Fungi
Chitin
Hyphae
cephalosporin C

Keywords

  • Acremonium chrysogenum
  • Allosamidin
  • Cephalosporin-C production
  • Chitinase
  • Fragmentation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

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title = "Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus",
abstract = "The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47{\%} of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76{\%} of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate. Copyright (C) 1998 Federation of European Microbiological Societies Published by Elsevier Scienced B.V. All rights reserved.",
keywords = "Acremonium chrysogenum, Allosamidin, Cephalosporin-C production, Chitinase, Fragmentation",
author = "E. S{\'a}ndor and T. Pusztahelyi and L. Karaffa and Z. Kar{\'a}nyi and Imre P{\'o}csi and S{\'a}ndor Bir{\'o} and A. Szentirmai and I. P{\'o}csi",
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T1 - Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus

AU - Sándor, E.

AU - Pusztahelyi, T.

AU - Karaffa, L.

AU - Karányi, Z.

AU - Pócsi, Imre

AU - Biró, Sándor

AU - Szentirmai, A.

AU - Pócsi, I.

PY - 1998/7/15

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N2 - The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate. Copyright (C) 1998 Federation of European Microbiological Societies Published by Elsevier Scienced B.V. All rights reserved.

AB - The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate. Copyright (C) 1998 Federation of European Microbiological Societies Published by Elsevier Scienced B.V. All rights reserved.

KW - Acremonium chrysogenum

KW - Allosamidin

KW - Cephalosporin-C production

KW - Chitinase

KW - Fragmentation

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