Alleviated mucosal and neuronal damage in a rat model of Crohn's disease

Petra Talapka, Lajos István Nagy, Alexra Pál, Marietta Zita Poles, A. Berkó, Mária Bagyánszki, L. Puskás, Éva Fekete, Nikolett Bódi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

AIM: To establish a rat model suitable to investigate the repetitive relapsing inflammations (RRI) characteristic to Crohn's disease.

METHODS: Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid (TNBS). RRI were mimicked by repeating administrations of TNBS. Tissue samples were taken from control, once, twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation. The means of the ulcerated area were measured to evaluate the macroscopic mucosal damage. The density of myenteric neurons was determined on whole mounts by HuC/HuD immunohistochemistry. Heme oxygenase-1 (HO-1) expression was evaluated by molecular biological techniques.

RESULTS: TNBS-treated rats displayed severe colitis, but the mortality was negligible, and an increase of body weight was characteristic throughout the experimental period. The widespread loss of myenteric neurons, and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration. After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased, and the neuronal loss was on a smaller scale and was limited to the inflamed area. HO-1 mRNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined. Nevertheless, the HO-1 protein up-regulation after the second TNBS treatment proved to be transient. Following the third treatment HO-1 protein expression could not be detected.

CONCLUSION: Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage. The persistent up-regulation of HO-1 mRNA expression may correlate with this.

Original languageEnglish
Pages (from-to)16690-16697
Number of pages8
JournalWorld Journal of Gastroenterology
Volume20
Issue number44
DOIs
Publication statusPublished - Nov 28 2014

Fingerprint

Trinitrobenzenesulfonic Acid
Heme Oxygenase-1
Crohn Disease
Inflammation
Up-Regulation
Colitis
Neurons
Messenger RNA
Proteins
Immunohistochemistry
Body Weight
Mortality
Therapeutics

Keywords

  • Crohn's disease
  • Experimental rat model
  • Heme oxygenase-1
  • Myenteric neurons
  • Repetitive relapsing inflammation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Talapka, P., Nagy, L. I., Pál, A., Poles, M. Z., Berkó, A., Bagyánszki, M., ... Bódi, N. (2014). Alleviated mucosal and neuronal damage in a rat model of Crohn's disease. World Journal of Gastroenterology, 20(44), 16690-16697. https://doi.org/10.3748/wjg.v20.i44.16690

Alleviated mucosal and neuronal damage in a rat model of Crohn's disease. / Talapka, Petra; Nagy, Lajos István; Pál, Alexra; Poles, Marietta Zita; Berkó, A.; Bagyánszki, Mária; Puskás, L.; Fekete, Éva; Bódi, Nikolett.

In: World Journal of Gastroenterology, Vol. 20, No. 44, 28.11.2014, p. 16690-16697.

Research output: Contribution to journalArticle

Talapka, P, Nagy, LI, Pál, A, Poles, MZ, Berkó, A, Bagyánszki, M, Puskás, L, Fekete, É & Bódi, N 2014, 'Alleviated mucosal and neuronal damage in a rat model of Crohn's disease', World Journal of Gastroenterology, vol. 20, no. 44, pp. 16690-16697. https://doi.org/10.3748/wjg.v20.i44.16690
Talapka, Petra ; Nagy, Lajos István ; Pál, Alexra ; Poles, Marietta Zita ; Berkó, A. ; Bagyánszki, Mária ; Puskás, L. ; Fekete, Éva ; Bódi, Nikolett. / Alleviated mucosal and neuronal damage in a rat model of Crohn's disease. In: World Journal of Gastroenterology. 2014 ; Vol. 20, No. 44. pp. 16690-16697.
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AU - Nagy, Lajos István

AU - Pál, Alexra

AU - Poles, Marietta Zita

AU - Berkó, A.

AU - Bagyánszki, Mária

AU - Puskás, L.

AU - Fekete, Éva

AU - Bódi, Nikolett

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KW - Experimental rat model

KW - Heme oxygenase-1

KW - Myenteric neurons

KW - Repetitive relapsing inflammation

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