All-or-Nothing Type Biphasic Cytokine Production of Human Lymphocytes After Exposure to Alzheimer's β-Amyloid Peptide

Antônio L. Teixeira, Helton J. Reis, Fernanda M. Coelho, Daniel S. Carneiro, Mauro M. Teixeira, Luciene B. Vieira, Marat A. Mukhamedyarov, Andrey L. Zefirov, Zoltán Janka, András Palotás

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17 Citations (Scopus)

Abstract

Background: Neuro-inflammation, triggered by β-amyloid peptide, is implicated as one of the primary contributors to Alzheimer's disease (AD) pathogenesis, and several cytokines were identified as key instigating factors. Methods: To reveal the inflammatory response of lymphocytes to the neuro-toxic β-amyloid peptide, we evaluated the release of several cytokines from peripheral blood mononuclear cells with immuno-assays (ELISA). From hyper-acute to chronic effects of β-amyloid peptide were assessed at a wide range of concentrations. Results: The pro-inflammatory interleukin (IL)-1β, tumor necrosis factor-α, monocyte chemotactic protein-1, and Rantes (acronym for regulated on activation, normal T-cell expressed and secreted) as well as the pleiotropic IL-6 showed a biphasic release pattern over time in both low and high doses of amyloid treatment: after an initial increase, their concentration gradually fell to the baseline. The suppressors IL-4 and IL-10 had a sinus-like secretion panel: an acute increase in their levels turned to a depression and later followed by their over-secretion. Interestingly, β-amyloid below 10-8 mol/L produced no effect at all, but any molarity above this threshold caused the very same cytokine secretion pattern, the mark of an all-or-nothing response of β-amyloid peptide. Conclusions: These results delineate a highly organized pro- and anti-inflammatory response of cells to the neuro-toxic peptide. This is the first study to describe how the β-amyloid-induced inflammatory processes in Alzheimer's dementia are regulated.

Original languageEnglish
Pages (from-to)891-895
Number of pages5
JournalBiological Psychiatry
Volume64
Issue number10
DOIs
Publication statusPublished - Nov 15 2008

Keywords

  • Alzheimer's disease
  • cytokine
  • interleukin
  • lymphocyte
  • β-amyloid peptide

ASJC Scopus subject areas

  • Biological Psychiatry

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    Teixeira, A. L., Reis, H. J., Coelho, F. M., Carneiro, D. S., Teixeira, M. M., Vieira, L. B., Mukhamedyarov, M. A., Zefirov, A. L., Janka, Z., & Palotás, A. (2008). All-or-Nothing Type Biphasic Cytokine Production of Human Lymphocytes After Exposure to Alzheimer's β-Amyloid Peptide. Biological Psychiatry, 64(10), 891-895. https://doi.org/10.1016/j.biopsych.2008.07.019