Alfalfa cyclins: Differential expression during the cell cycle and in plant organs

Heribert Hirt, Mátyás Mink, Martin Pfosser, Laszlo Bögre, J. Györgyey, Claudia Jonak, Anton Gartner, D. Dudits, Erwin Heberle-Bors

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Cell division in eukaryotes is mediated by the action of the mitosis promoting factor, which is composed of the CDC2 protein kinase and one of the various mitotic cyclins. We have recently isolated a cdc2 gene from alfalfa. Here, we report the isolation of two cyclin genes, cycMs1 and cycMs2, from alfalfa. The cycMs2 gene shows highest similarity to type B cyclins. In contrast, the predicted amino acid sequence of the cycMs1 gene shows similar homology scores to cyclins of all types (25 to 35%). Both genes are expressed in dividing suspension cultured cells but cease to be expressed when the cells enter stationary phase. In synchronized alfalfa suspension cultured cells, the mRNAs of cycMs1 and cycMs2 show maximal expression in the G2 and M phases. Transcripts of cycMs2 are found only in late G2 and M phase cells, an expression pattern typical for cyclin B genes, whereas cycMs1 appears with the onset of G2. This pattern indicates that alfalfa cycMs1 and cycMs2 belong to different classes of cyclins. In young leaves, expression of both genes is high, whereas in mature leaves no transcripts can be detected, indicating that the two cyclin genes are true cell division markers at the mRNA level. In other organs, a more complex expression pattern of the two cyclin genes was found.

Original languageEnglish
Pages (from-to)1531-1538
Number of pages8
JournalPlant Cell
Volume4
Issue number12
Publication statusPublished - Dec 1992

Fingerprint

Cyclins
Medicago sativa
cyclins
plant organs
alfalfa
cell cycle
Cell Cycle
Genes
Cells
Cell Division
genes
mitosis
Cyclin B
G2 Phase
interphase
cultured cells
cell division
Cultured Cells
Suspensions
Maturation-Promoting Factor

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Hirt, H., Mink, M., Pfosser, M., Bögre, L., Györgyey, J., Jonak, C., ... Heberle-Bors, E. (1992). Alfalfa cyclins: Differential expression during the cell cycle and in plant organs. Plant Cell, 4(12), 1531-1538.

Alfalfa cyclins : Differential expression during the cell cycle and in plant organs. / Hirt, Heribert; Mink, Mátyás; Pfosser, Martin; Bögre, Laszlo; Györgyey, J.; Jonak, Claudia; Gartner, Anton; Dudits, D.; Heberle-Bors, Erwin.

In: Plant Cell, Vol. 4, No. 12, 12.1992, p. 1531-1538.

Research output: Contribution to journalArticle

Hirt, H, Mink, M, Pfosser, M, Bögre, L, Györgyey, J, Jonak, C, Gartner, A, Dudits, D & Heberle-Bors, E 1992, 'Alfalfa cyclins: Differential expression during the cell cycle and in plant organs', Plant Cell, vol. 4, no. 12, pp. 1531-1538.
Hirt H, Mink M, Pfosser M, Bögre L, Györgyey J, Jonak C et al. Alfalfa cyclins: Differential expression during the cell cycle and in plant organs. Plant Cell. 1992 Dec;4(12):1531-1538.
Hirt, Heribert ; Mink, Mátyás ; Pfosser, Martin ; Bögre, Laszlo ; Györgyey, J. ; Jonak, Claudia ; Gartner, Anton ; Dudits, D. ; Heberle-Bors, Erwin. / Alfalfa cyclins : Differential expression during the cell cycle and in plant organs. In: Plant Cell. 1992 ; Vol. 4, No. 12. pp. 1531-1538.
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