Aleppo tannin: structural analysis and salivary amylase inhibition

Ágnes Zajácz, G. Gyémánt, Natale Vittori, L. Kandra

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effectiveness and specificity of a tannin inhibition on human salivary amylase (HSA) catalyzed hydrolysis was studied using 2-chloro-4-nitrophenyl 4-O-β-d-galactopyranosyl-α-maltoside (GalG2-CNP) and amylose substrates. Aleppo tannin was isolated from the gall nut of Aleppo oak. This tannin is a gallotannin, in which glucose is esterified with gallic acids. This is the first kinetic report, which details the inhibitory effects of this compound on HSA. A mixed non-competitive type inhibition has been observed on both substrates. The extent of inhibition is markedly dependent on the substrate-type. Kinetic constants were calculated from Lineweaver-Burk secondary plots for GalG2-CNP (KEI 0.82 μg mL-1, KESI 3.3 μg mL-1). This indicates a 1:1 binding ratio of inhibitor-enzyme and/or inhibitor-enzyme-substrate complex. When amylose was the substrate the binding ratio of inhibitor to enzyme-substrate complex was found to be 2:1, with the binding constants of KEI 17.4 μg mL-1, KESI 14.9 μg mL-1, KESI2 9.6 μg mL-1. Presumably, the tannin inhibitor can bind not only to HSA, but to the amylose substrate, as well. Kinetic data suggest that Aleppo tannin is a more efficient amylase inhibitor than the recently studied other tannin with quinic acid core (GalG2-CNP: KEI 9.0 μg mL-1, KESI 47.9 μg mL-1).

Original languageEnglish
Pages (from-to)717-723
Number of pages7
JournalCarbohydrate Research
Volume342
Issue number5
DOIs
Publication statusPublished - Apr 9 2007

Fingerprint

Tannins
Amylases
Structural analysis
Amylose
Enzyme Inhibitors
Substrates
Quinic Acid
Hydrolyzable Tannins
Gallic Acid
Nuts
Kinetics
Hydrolysis
Glucose
aleppo tannin
Enzymes

Keywords

  • Aleppo tannin
  • Gallotannin
  • HSA
  • Inhibitor
  • Kinetic analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry

Cite this

Aleppo tannin : structural analysis and salivary amylase inhibition. / Zajácz, Ágnes; Gyémánt, G.; Vittori, Natale; Kandra, L.

In: Carbohydrate Research, Vol. 342, No. 5, 09.04.2007, p. 717-723.

Research output: Contribution to journalArticle

Zajácz, Ágnes ; Gyémánt, G. ; Vittori, Natale ; Kandra, L. / Aleppo tannin : structural analysis and salivary amylase inhibition. In: Carbohydrate Research. 2007 ; Vol. 342, No. 5. pp. 717-723.
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