AIDS treatment and the heat shock protein level in the gastrointestinal tract

P. Csere, G. Varbiro, B. Sümegi, G. Mózsik

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

AIDS has several manifestations in the gastrointestinal tract - 2',3'-dideoxycytidine (ddC) as an anti-AIDS drug will also produce some effects on the alimentary tract: it causes inflammation. The analysis of the expression of the heat shock proteins (hsp60 and hsp70) is a new marker of the inflammation caused by external stress. The hsp60 is located mainly in the mitochondria; hsp70 is found in both cytoplasm and the mitochondria. The aim of the present study was to reveal whether ddC can produce any expressive biochemical changes in the oral cavity or generally in the gastrointestinal tract. Methods. The rats were treated with intraperitoneal administration of 1 mg ddC/day for two weeks. The animals were killed by cervical dislocation, then the buccal, lingual, oesophageal, gastric, duodenal and colon mucosae were removed, and frozen in liquid nitrogen. Protein extract was obtained and separated by SDS-PAGE, followed by the Western blotting of the proteins to the nitrocellulose membrane. After binding the first antibody (mouse-derived anti-heat shock protein), a second antibody was administered (anti-mouse IgG, labelled with peroxidase). The antigen-antibody reaction was detected by 3,3'-diaminobenzidine tetrahydrochloride dihydrate. Results. 1. In the control and in the treated groups, the hsp60 monoclonal antibody binding was visible in the oesophageal, gastric, duodenal and colon mucosae, but, in the buccal and lingual mucosae, it was not detectable in the treated group. 2. The hsp70 was detectable in every tissue of both treated and non-treated groups. To verify whether the cause was indirect oxidative damage, the NADH-dihydrogenase (complex I), cytochrome-oxidoreductase (complex III) and cytochrome-oxidase (complex IV), members of the respiratory chain, were examined but no alterations were found. Our results indicate that the disappearance of hsp60 ih the oral cavity is a unique phenomenon following ddC treatment. This damage might be caused by the ddC interaction with hsp60; however, the exact mechanism is not yet known.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalInflammopharmacology
Volume5
Issue number1
Publication statusPublished - 1997

Fingerprint

Zalcitabine
Heat-Shock Proteins
Gastrointestinal Tract
Acquired Immunodeficiency Syndrome
Tongue
Mouth
Stomach
Mitochondria
Colon
Mucous Membrane
3,3'-Diaminobenzidine
Antigen-Antibody Reactions
Inflammation
Anti-HIV Agents
Collodion
Antibodies
Cheek
Electron Transport Complex III
Mouth Mucosa
Electron Transport Complex IV

Keywords

  • AIDS
  • dideoxycytidine
  • heat shock protein
  • inflammation

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

AIDS treatment and the heat shock protein level in the gastrointestinal tract. / Csere, P.; Varbiro, G.; Sümegi, B.; Mózsik, G.

In: Inflammopharmacology, Vol. 5, No. 1, 1997, p. 83-91.

Research output: Contribution to journalArticle

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