Agrobacterium tumefaciens-mediated transformation of the zygomycete fungus Backusella lamprospora

Ildikó Nyilasi, T. Papp, Árpád Csernetics, C. Vágvölgyi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Agrobacterium-mediated transformation was adapted to Backusella lamprospora, a zygomycete fungus closely related to Mucor. The transforming plasmid contained the hygromycin B resistance (hph) and the green fluorescent protein (gfp) genes under the control of the regulator regions of the Mucor circinelloides gpd1 gene. The presence of the hph and gfp genes in the transformants was detected by PCR. The introduced genes could also be amplified directly from the spores of the transformants. The transformation efficiency was investigated by fluorescence microscopy of the transformed spores. A gradual decrease in the hygromycin B resistance was observed during several cultivation cycles: the growth of the transformants on the selection medium became slower, and the detection of the introduced gene became more difficult.

Original languageEnglish
Pages (from-to)59-64
Number of pages6
JournalJournal of Basic Microbiology
Volume48
Issue number1
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Agrobacterium tumefaciens
Fungi
Hygromycin B
Mucor
Genes
Green Fluorescent Proteins
Spores
Agrobacterium
Fluorescence Microscopy
Plasmids
Polymerase Chain Reaction
Growth

Keywords

  • Backusella
  • Genetic transformation
  • Green fluorescent protein
  • Hygromycin B
  • Mucor
  • Mucorales

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Agrobacterium tumefaciens-mediated transformation of the zygomycete fungus Backusella lamprospora. / Nyilasi, Ildikó; Papp, T.; Csernetics, Árpád; Vágvölgyi, C.

In: Journal of Basic Microbiology, Vol. 48, No. 1, 02.2008, p. 59-64.

Research output: Contribution to journalArticle

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