Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides

I. Nyilasi, K. Ács, T. Papp, E. Nagy, C. Vágvölgyi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalFolia Microbiologica
Volume50
Issue number5
DOIs
Publication statusPublished - Dec 1 2005

    Fingerprint

ASJC Scopus subject areas

  • Microbiology

Cite this