Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides

I. Nyilasi, K. Ács, T. Papp, E. Nagy, C. Vágvölgyi

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20 Citations (Scopus)

Abstract

The Agrobacterium tumefaciens-mediated transformation of the zygomycetous fungus Mucor circinelloides is described. A method was also developed for the hygromycin B-based selection of Mucor transformants. Transformation with the hygromycin B phosphotransferase gene of Escherichia coli controlled by the heterologous Aspergillus nidulans trpC promoter resulted in hygromycin B-resistant clones. The presence of the hygromycin resistance gene in the genome of the transformants was verified by polymerase chain reaction and Southern hybridization: the latter analyses revealed integrations in the host genome at different sites in different transformants. The stability of transformants remained questionable during the latter analyses.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalFolia Microbiologica
Volume50
Issue number5
DOIs
Publication statusPublished - 2005

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Hygromycin B
Mucor
Agrobacterium tumefaciens
hygromycin-B kinase
Genome
Aspergillus nidulans
Genes
Fungi
Clone Cells
Escherichia coli
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology

Cite this

Agrobacterium tumefaciens-mediated transformation of Mucor circinelloides. / Nyilasi, I.; Ács, K.; Papp, T.; Nagy, E.; Vágvölgyi, C.

In: Folia Microbiologica, Vol. 50, No. 5, 2005, p. 415-420.

Research output: Contribution to journalArticle

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