Agrobacterium rhizogenes-mediated transformation of soybean to study root biology

Attila Kereszt, Dongxue Li, Arief Indrasumunar, Cuc D.T. Nguyen, Sureeporn Nontachaiyapoom, Mark Kinkema, Peter M. Gresshoff

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

This protocol is used to induce transgenic roots on soybean to study the function of genes required in biological processes of the root. Young seedlings with unfolded cotyledons are infected at the cotyledonary node and/or hypocotyl with Agrobacterium rhizogenes carrying the gene construct to be tested and the infection sites are kept in an environment of high humidity. When the emerged hairy roots can support the plants, the main roots are removed and the transgenic roots can be tested. Using this method, almost 100% of the infected plants form hairy roots within 1 month from the start of the experiments.

Original languageEnglish
Pages (from-to)948-952
Number of pages5
JournalNature Protocols
Volume2
Issue number4
DOIs
Publication statusPublished - Apr 1 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Kereszt, A., Li, D., Indrasumunar, A., Nguyen, C. D. T., Nontachaiyapoom, S., Kinkema, M., & Gresshoff, P. M. (2007). Agrobacterium rhizogenes-mediated transformation of soybean to study root biology. Nature Protocols, 2(4), 948-952. https://doi.org/10.1038/nprot.2007.141