Agrobacterium-mediated genetic transformation of an interspecific grapevine

J. Mozsár, O. Viczián, S. Süle

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23 Citations (Scopus)

Abstract

Anther-derived somatic embryos (cv. Georgikon 28) were transformed with reporter gene encoding β-glucuronidase, using the Agrobacterium tumefaciens strain EIIA101. Embryogenic calli were maintained and proliferated on MS medium containing plant growth regulators and 50 mg · l-1 kanamycin. Numerous somatic embryos were obtained by placing calli on hormone-free MS medium supplemented with 50 mg · l-1 kanamycin. Transgenic plants were regenerated from these embryos. Application of antioxidants during and after cocultivation reduced necrotic symptoms of plant cells, while in the presence of an antioxidant during cocultivation the frequency of transformation was decreased. Injuries caused by particle bombardment did not increase the transformation efficiency.

Original languageEnglish
Pages (from-to)127-130
Number of pages4
JournalVitis
Volume37
Issue number3
Publication statusPublished - Sep 1 1998

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Keywords

  • Agrobacterium tumefaciens
  • Antioxidant
  • Biolistic bombardment
  • Genetic transformation
  • Somatic embryogenesis

ASJC Scopus subject areas

  • Genetics
  • Horticulture

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