Aging of Penicillium chrysogenum cultures under carbon starvation

II: Protease and N-acetyl-β-D-hexosaminidase production

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Abstract

In aging carbon-depleted cultures of Penicillium chrysogenum the fragmentation and autolysis of old mycelia were coupled with the production of high levels of extracellular protease and N-acetyl-β-D-hexosaminidase; this could be stopped by the addition of an extra dose of glucose at any incubation time tested, but was not affected by endogenous NH3. After the addition of glucose, intracellular enzyme accumulation was observed only for the N-acetyl-β-D-hexosaminidase, and the concomitant decrease in both the extracellular protease and N-acetyl-β-D-hexosaminidase activities was not caused by the action of extracellular proteases. The physiological function of the N-acetyl-β-D-hexosaminidase remains to be elucidated because the P. chrysogenum culture studied did not utilize N-acetyl-D-glucosamine as a carbon source. However, the proteases (mainly serine and, to a much smaller extent, metalloproteases) might provide surviving hyphal fragments with sufficient amino acids to maintain the cryptic growth observed in aging carbon-depleted cultures.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalBiotechnology and Applied Biochemistry
Volume25
Issue number1
Publication statusPublished - 1997

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Penicillium chrysogenum
Hexosaminidases
Starvation
Carbon
Aging of materials
Glucose
Glucosamine
Autolysis
Acetylglucosamine
Mycelium
Metalloproteases
Serine Proteases
Amino acids
Peptide Hydrolases
Enzymes
Amino Acids
protease N
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

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title = "Aging of Penicillium chrysogenum cultures under carbon starvation: II: Protease and N-acetyl-β-D-hexosaminidase production",
abstract = "In aging carbon-depleted cultures of Penicillium chrysogenum the fragmentation and autolysis of old mycelia were coupled with the production of high levels of extracellular protease and N-acetyl-β-D-hexosaminidase; this could be stopped by the addition of an extra dose of glucose at any incubation time tested, but was not affected by endogenous NH3. After the addition of glucose, intracellular enzyme accumulation was observed only for the N-acetyl-β-D-hexosaminidase, and the concomitant decrease in both the extracellular protease and N-acetyl-β-D-hexosaminidase activities was not caused by the action of extracellular proteases. The physiological function of the N-acetyl-β-D-hexosaminidase remains to be elucidated because the P. chrysogenum culture studied did not utilize N-acetyl-D-glucosamine as a carbon source. However, the proteases (mainly serine and, to a much smaller extent, metalloproteases) might provide surviving hyphal fragments with sufficient amino acids to maintain the cryptic growth observed in aging carbon-depleted cultures.",
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T1 - Aging of Penicillium chrysogenum cultures under carbon starvation

T2 - II: Protease and N-acetyl-β-D-hexosaminidase production

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AU - Pócsi, I.

AU - Szentirmai, A.

PY - 1997

Y1 - 1997

N2 - In aging carbon-depleted cultures of Penicillium chrysogenum the fragmentation and autolysis of old mycelia were coupled with the production of high levels of extracellular protease and N-acetyl-β-D-hexosaminidase; this could be stopped by the addition of an extra dose of glucose at any incubation time tested, but was not affected by endogenous NH3. After the addition of glucose, intracellular enzyme accumulation was observed only for the N-acetyl-β-D-hexosaminidase, and the concomitant decrease in both the extracellular protease and N-acetyl-β-D-hexosaminidase activities was not caused by the action of extracellular proteases. The physiological function of the N-acetyl-β-D-hexosaminidase remains to be elucidated because the P. chrysogenum culture studied did not utilize N-acetyl-D-glucosamine as a carbon source. However, the proteases (mainly serine and, to a much smaller extent, metalloproteases) might provide surviving hyphal fragments with sufficient amino acids to maintain the cryptic growth observed in aging carbon-depleted cultures.

AB - In aging carbon-depleted cultures of Penicillium chrysogenum the fragmentation and autolysis of old mycelia were coupled with the production of high levels of extracellular protease and N-acetyl-β-D-hexosaminidase; this could be stopped by the addition of an extra dose of glucose at any incubation time tested, but was not affected by endogenous NH3. After the addition of glucose, intracellular enzyme accumulation was observed only for the N-acetyl-β-D-hexosaminidase, and the concomitant decrease in both the extracellular protease and N-acetyl-β-D-hexosaminidase activities was not caused by the action of extracellular proteases. The physiological function of the N-acetyl-β-D-hexosaminidase remains to be elucidated because the P. chrysogenum culture studied did not utilize N-acetyl-D-glucosamine as a carbon source. However, the proteases (mainly serine and, to a much smaller extent, metalloproteases) might provide surviving hyphal fragments with sufficient amino acids to maintain the cryptic growth observed in aging carbon-depleted cultures.

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