Aging and oxidized proteins

Generation and degradation

Sataro Goto, Ryoya Takahashi, Hideko Nakamoto

Research output: Contribution to journalArticle

Abstract

It has been suggested that reactive oxygen species (ROS) are involved in the generation of altered proteins that are reported to increase with advancing age. Among chemical modifications of proteins, carbonyls are their useful markers that can be determined and characterized by the spectroscopic and immunological analyses of protein samples derivatized with the carbonyl reagent 2,4-dinitrophenylhydrazine. The age-associated accumulation of altered proteins can be caused not only by the age- related increase in ROS generation, but also by the decline of protein degradation. In fact, half-lives of proteins including oxidatively modified proteins in cells from old mice were shown to be significantly longer than those in cells from young mice. A decrease in proteasome activity appears to be primarily responsible for an age-related decline in the degradation of oxidatively modified proteins. Importantly, proteasome itself was suggested to alter with age by its decreased specific activity (die activity per intensity of immunological signals for die subunits), thus likely contributing to form a vicious cycle of accumulation of altered proteins.

Original languageEnglish
Pages (from-to)53-61
Number of pages9
JournalJournal of Clinical Biochemistry and Nutrition
Volume35
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

Proteolysis
Aging of materials
Degradation
degradation
Proteins
proteins
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
reactive oxygen species
Reactive Oxygen Species
mice
protein degradation
Chemical modification
half life
cells

Keywords

  • Aging
  • Oxidative modification
  • Proteasome
  • Protein carbonyl
  • Protein degradation

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Aging and oxidized proteins : Generation and degradation. / Goto, Sataro; Takahashi, Ryoya; Nakamoto, Hideko.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 35, No. 2, 2004, p. 53-61.

Research output: Contribution to journalArticle

Goto, Sataro ; Takahashi, Ryoya ; Nakamoto, Hideko. / Aging and oxidized proteins : Generation and degradation. In: Journal of Clinical Biochemistry and Nutrition. 2004 ; Vol. 35, No. 2. pp. 53-61.
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