Age-related difference of site-specific histone modifications in rat liver

Kyojiro Kawakami, Akihiro Nakamura, Akihito Ishigami, S. Goto, Ryoya Takahashi

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Aging is associated with decrease in activities of the transcription, replication and DNA repair that can result in deterioration of cellular and tissue functions. Changes of chromatin structures with age are likely major underling mechanisms for the functional decline. Chromatin consists of DNA and histones as well as non-histone proteins. While age-associated change of DNA methylation is well documented, little information is available on site-specific histone modifications in aging. We studied here age-related change of selected modifications of rat liver histone, i.e., histone H3 Lys9 acetylation (H3K9ac), H3 Lys9 methylation (H3K9me), H3 Ser10 phosphorylation (H3S10ph) and H3 Lys14 acetylation (H3K14ac). H3K9ac was decreased and H3S10ph was increased with age significantly. In view of reports indicating that decrease in acetylation and increase in phosphorylation of H3 histones can suppress gene activity, our findings suggest that a mechanism of decreased chromatin functions with age is due to such epigenetic changes.

Original languageEnglish
Pages (from-to)415-421
Number of pages7
JournalBiogerontology
Volume10
Issue number4
DOIs
Publication statusPublished - 2009

Fingerprint

Histone Code
Histones
Acetylation
Chromatin
Liver
Phosphorylation
DNA Methylation
Epigenomics
DNA Repair
Methylation
DNA
Genes
Proteins

Keywords

  • Acetylation
  • Aging
  • Epigenetics
  • Histone modification
  • Methylation
  • Phosphorylation

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Gerontology
  • Ageing

Cite this

Age-related difference of site-specific histone modifications in rat liver. / Kawakami, Kyojiro; Nakamura, Akihiro; Ishigami, Akihito; Goto, S.; Takahashi, Ryoya.

In: Biogerontology, Vol. 10, No. 4, 2009, p. 415-421.

Research output: Contribution to journalArticle

Kawakami, K, Nakamura, A, Ishigami, A, Goto, S & Takahashi, R 2009, 'Age-related difference of site-specific histone modifications in rat liver', Biogerontology, vol. 10, no. 4, pp. 415-421. https://doi.org/10.1007/s10522-008-9176-0
Kawakami, Kyojiro ; Nakamura, Akihiro ; Ishigami, Akihito ; Goto, S. ; Takahashi, Ryoya. / Age-related difference of site-specific histone modifications in rat liver. In: Biogerontology. 2009 ; Vol. 10, No. 4. pp. 415-421.
@article{ce5e0465acd94ff59d3cc9611caecc27,
title = "Age-related difference of site-specific histone modifications in rat liver",
abstract = "Aging is associated with decrease in activities of the transcription, replication and DNA repair that can result in deterioration of cellular and tissue functions. Changes of chromatin structures with age are likely major underling mechanisms for the functional decline. Chromatin consists of DNA and histones as well as non-histone proteins. While age-associated change of DNA methylation is well documented, little information is available on site-specific histone modifications in aging. We studied here age-related change of selected modifications of rat liver histone, i.e., histone H3 Lys9 acetylation (H3K9ac), H3 Lys9 methylation (H3K9me), H3 Ser10 phosphorylation (H3S10ph) and H3 Lys14 acetylation (H3K14ac). H3K9ac was decreased and H3S10ph was increased with age significantly. In view of reports indicating that decrease in acetylation and increase in phosphorylation of H3 histones can suppress gene activity, our findings suggest that a mechanism of decreased chromatin functions with age is due to such epigenetic changes.",
keywords = "Acetylation, Aging, Epigenetics, Histone modification, Methylation, Phosphorylation",
author = "Kyojiro Kawakami and Akihiro Nakamura and Akihito Ishigami and S. Goto and Ryoya Takahashi",
year = "2009",
doi = "10.1007/s10522-008-9176-0",
language = "English",
volume = "10",
pages = "415--421",
journal = "Biogerontology",
issn = "1389-5729",
publisher = "Springer Netherlands",
number = "4",

}

TY - JOUR

T1 - Age-related difference of site-specific histone modifications in rat liver

AU - Kawakami, Kyojiro

AU - Nakamura, Akihiro

AU - Ishigami, Akihito

AU - Goto, S.

AU - Takahashi, Ryoya

PY - 2009

Y1 - 2009

N2 - Aging is associated with decrease in activities of the transcription, replication and DNA repair that can result in deterioration of cellular and tissue functions. Changes of chromatin structures with age are likely major underling mechanisms for the functional decline. Chromatin consists of DNA and histones as well as non-histone proteins. While age-associated change of DNA methylation is well documented, little information is available on site-specific histone modifications in aging. We studied here age-related change of selected modifications of rat liver histone, i.e., histone H3 Lys9 acetylation (H3K9ac), H3 Lys9 methylation (H3K9me), H3 Ser10 phosphorylation (H3S10ph) and H3 Lys14 acetylation (H3K14ac). H3K9ac was decreased and H3S10ph was increased with age significantly. In view of reports indicating that decrease in acetylation and increase in phosphorylation of H3 histones can suppress gene activity, our findings suggest that a mechanism of decreased chromatin functions with age is due to such epigenetic changes.

AB - Aging is associated with decrease in activities of the transcription, replication and DNA repair that can result in deterioration of cellular and tissue functions. Changes of chromatin structures with age are likely major underling mechanisms for the functional decline. Chromatin consists of DNA and histones as well as non-histone proteins. While age-associated change of DNA methylation is well documented, little information is available on site-specific histone modifications in aging. We studied here age-related change of selected modifications of rat liver histone, i.e., histone H3 Lys9 acetylation (H3K9ac), H3 Lys9 methylation (H3K9me), H3 Ser10 phosphorylation (H3S10ph) and H3 Lys14 acetylation (H3K14ac). H3K9ac was decreased and H3S10ph was increased with age significantly. In view of reports indicating that decrease in acetylation and increase in phosphorylation of H3 histones can suppress gene activity, our findings suggest that a mechanism of decreased chromatin functions with age is due to such epigenetic changes.

KW - Acetylation

KW - Aging

KW - Epigenetics

KW - Histone modification

KW - Methylation

KW - Phosphorylation

UR - http://www.scopus.com/inward/record.url?scp=67649669224&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649669224&partnerID=8YFLogxK

U2 - 10.1007/s10522-008-9176-0

DO - 10.1007/s10522-008-9176-0

M3 - Article

VL - 10

SP - 415

EP - 421

JO - Biogerontology

JF - Biogerontology

SN - 1389-5729

IS - 4

ER -