Age-related change in the amount of ubiquitinated histones in the mouse brain

Shin ichi Morimoto, Shozo Komatsu, Ryoya Takahashi, Mitsuyoshi Matsuo, S. Goto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated age-related changes in the ubiquitination of histones in mouse brains. By Western blot analysis using rabbit anti-ubiquitin antibodies, histone H2A and H2B were found to be the only proteins that are ubiquitinated in the acid-extractable nuclear proteins of both young (4-month-old) and senescent (29-month-old) mouse brains. The proportion of ubiquitinated histones was about 30% higher in senescent mice than in young ones. When total brain homogenates were analyzed similarly using somewhat different age groups of the animals, the proportion in the old animals was about 2-fold higher than that in the young.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalArchives of Gerontology and Geriatrics
Volume16
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

Histones
brain
Brain
animal
Ubiquitinated Proteins
Ubiquitination
Nuclear Proteins
Ubiquitin
age group
Anti-Idiotypic Antibodies
Age Groups
Western Blotting
Rabbits
Acids

Keywords

  • Aging
  • Mouse brain
  • Ubiquitin
  • Ubiquitinated histone

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Age-related change in the amount of ubiquitinated histones in the mouse brain. / Morimoto, Shin ichi; Komatsu, Shozo; Takahashi, Ryoya; Matsuo, Mitsuyoshi; Goto, S.

In: Archives of Gerontology and Geriatrics, Vol. 16, No. 3, 1993, p. 217-224.

Research output: Contribution to journalArticle

Morimoto, Shin ichi ; Komatsu, Shozo ; Takahashi, Ryoya ; Matsuo, Mitsuyoshi ; Goto, S. / Age-related change in the amount of ubiquitinated histones in the mouse brain. In: Archives of Gerontology and Geriatrics. 1993 ; Vol. 16, No. 3. pp. 217-224.
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