Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans

Marta Kinga Lemieszek, Marco Chilosi, Marcin Golec, Czesława Skórska, A. Dinnyés, Kaveh Mashayekhi, Klemens Vierlinger, Francois Huaux, Matthias Wielscher, Manuela Hofner, Yousof Yakoub, Chiara Pastena, Isabella Daniele, Grazyna Cholewa, Jolanta Sitkowska, Wiesława Lisowska, Jacek Zwoliński, Janusz Milanowski, Barbara Mackiewicz, Anna Góra-Florek & 2 others Rolf Ziesche, Jacek Dutkiewicz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hypersensitivity pneumonitis (HP) represents the immunologically mediated lung disease induced by repeated inhalations of a wide variety of certain finely dispersed organic antigens. In susceptible subjects, these inhalations provoke a hypersensitivity reaction characterized by intense inflammation of the terminal bronchioles, the interstitium and the alveolar tree. The inflammation often organizes into granulomas and may progress to pulmonary fibrosis. Our previous work indicated that cell extract of gram-negative bacteria Pantoea agglomerans (SE-PA) causes, in young C57BL/6J mice, pulmonary changes that are very similar to the clinical manifestations of HP in men. The purpose of presented studies was to describe the response of mice immune system while exposed to SE-PA. Particular attention was paid to examine the age influence on SE-PA induced inflammation and fibrosis in lung tissue. We used 3- and 18-month-old C57BL/6J mice. Lung samples were collected from untreated mice and animals exposed to harmful agent for 7 and 28 days. HP development was monitored by histological and biochemical evaluation. Using ELISA tests, we examined concentration of pro- and anti-inflammatory cytokines in lung homogenates. Our study demonstrated again that SE-PA provokes in mice changes typical for the clinical picture of HP, and that successive stages of disease (acute, subacute and chronic) might be obtained by modulation of time exposure. Furthermore, we found that animals' age at the time of sensitization influences the nature of observed changes (cytokine expression pattern) and the final outcome (reaction intensity and scale of fibrosis).

Original languageEnglish
Pages (from-to)640-650
Number of pages11
JournalInhalation Toxicology
Volume25
Issue number11
DOIs
Publication statusPublished - Sep 2013

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Pantoea
Extrinsic Allergic Alveolitis
Animals
Cytokines
Lung
Pulmonary diseases
Immune system
Inflammation
Inbred C57BL Mouse
Inhalation
Bacteria
Fibrosis
Anti-Inflammatory Agents
Modulation
Tissue
Bronchioles
Antigens
Pulmonary Fibrosis
Acute Disease
Cell Extracts

Keywords

  • Hypersensitivity pneumonitis
  • Mouse model
  • Pantoea agglomerans

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Lemieszek, M. K., Chilosi, M., Golec, M., Skórska, C., Dinnyés, A., Mashayekhi, K., ... Dutkiewicz, J. (2013). Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans. Inhalation Toxicology, 25(11), 640-650. https://doi.org/10.3109/08958378.2013.827284

Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans. / Lemieszek, Marta Kinga; Chilosi, Marco; Golec, Marcin; Skórska, Czesława; Dinnyés, A.; Mashayekhi, Kaveh; Vierlinger, Klemens; Huaux, Francois; Wielscher, Matthias; Hofner, Manuela; Yakoub, Yousof; Pastena, Chiara; Daniele, Isabella; Cholewa, Grazyna; Sitkowska, Jolanta; Lisowska, Wiesława; Zwoliński, Jacek; Milanowski, Janusz; Mackiewicz, Barbara; Góra-Florek, Anna; Ziesche, Rolf; Dutkiewicz, Jacek.

In: Inhalation Toxicology, Vol. 25, No. 11, 09.2013, p. 640-650.

Research output: Contribution to journalArticle

Lemieszek, MK, Chilosi, M, Golec, M, Skórska, C, Dinnyés, A, Mashayekhi, K, Vierlinger, K, Huaux, F, Wielscher, M, Hofner, M, Yakoub, Y, Pastena, C, Daniele, I, Cholewa, G, Sitkowska, J, Lisowska, W, Zwoliński, J, Milanowski, J, Mackiewicz, B, Góra-Florek, A, Ziesche, R & Dutkiewicz, J 2013, 'Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans', Inhalation Toxicology, vol. 25, no. 11, pp. 640-650. https://doi.org/10.3109/08958378.2013.827284
Lemieszek, Marta Kinga ; Chilosi, Marco ; Golec, Marcin ; Skórska, Czesława ; Dinnyés, A. ; Mashayekhi, Kaveh ; Vierlinger, Klemens ; Huaux, Francois ; Wielscher, Matthias ; Hofner, Manuela ; Yakoub, Yousof ; Pastena, Chiara ; Daniele, Isabella ; Cholewa, Grazyna ; Sitkowska, Jolanta ; Lisowska, Wiesława ; Zwoliński, Jacek ; Milanowski, Janusz ; Mackiewicz, Barbara ; Góra-Florek, Anna ; Ziesche, Rolf ; Dutkiewicz, Jacek. / Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomerans. In: Inhalation Toxicology. 2013 ; Vol. 25, No. 11. pp. 640-650.
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AU - Dinnyés, A.

AU - Mashayekhi, Kaveh

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AU - Zwoliński, Jacek

AU - Milanowski, Janusz

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