Age-development and inducibility of hepatic glutathione S-transferase activities in mice, rats, rabbits and guinea-pigs

Z. Gregus, F. Varga, A. Schmelás

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

1. Hepatic glutathione S-transferase (GST) activities towards l-chloro-3,4-dinitrobenzene (DNCB), 3,4-dichloronitrobenzene (DCNB), sulfobromophthalein (BSP), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), trans-4-phenyl-3-buten-2-one (TPBO) and 1,2-epoxy-3-(p-nitrophenoxy)propane (ENPP) were determined in mice, rats, rabbits and guinea-pigs during ageing and after pretreatment with enzyme inducers. 2. Variations were observed (a) in the developmental patterns and (b) in the phenobarbital-, benzo(a)pyrene-, pregnenolone-16α-carbonitrile-, butylated hydroxyanisole-, trans-stilbene oxide-inducibility of hepatic GST activities in the same species towards different substrates. 3. For example, in rats GST activities for EA, DCNB and TPBO increased respectively, 2.3-, 4.8- and 25-fold during age-development, and after treatment with TSO 1.2-, 3.6- and 1.3-fold. 4. Species differences were found (a) in the maturation and (b) in the inducibility of GST activities. For instance, GST activity toward EA at birth is mature in guinea pigs but not in the other species; phenobarbital treatment increased GST activities in mice and rats but not in rabbits and guinea-pigs; treatment with trans-stilbene oxide enhanced GST activity for TPBO 4.5-fold in mice but not at all in rats. 5. It is concluded that hepatic glutathione conjugation exhibits functional heterogeneity which may be due to species dependent variations in the responsiveness of GST isoenzymes to endogenous and exogenous influences.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume80
Issue number1
DOIs
Publication statusPublished - 1985

Fingerprint

Glutathione Transferase
Guinea Pigs
Rabbits
Liver
Ethacrynic Acid
Phenobarbital
Pregnenolone Carbonitrile
Sulfobromophthalein
Butylated Hydroxyanisole
Dinitrochlorobenzene
Benzo(a)pyrene
Isoenzymes
Glutathione
Parturition
Enzymes
benzylideneacetone

ASJC Scopus subject areas

  • Immunology

Cite this

@article{6f33d89517c141529af5fd680fde0db9,
title = "Age-development and inducibility of hepatic glutathione S-transferase activities in mice, rats, rabbits and guinea-pigs",
abstract = "1. Hepatic glutathione S-transferase (GST) activities towards l-chloro-3,4-dinitrobenzene (DNCB), 3,4-dichloronitrobenzene (DCNB), sulfobromophthalein (BSP), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), trans-4-phenyl-3-buten-2-one (TPBO) and 1,2-epoxy-3-(p-nitrophenoxy)propane (ENPP) were determined in mice, rats, rabbits and guinea-pigs during ageing and after pretreatment with enzyme inducers. 2. Variations were observed (a) in the developmental patterns and (b) in the phenobarbital-, benzo(a)pyrene-, pregnenolone-16α-carbonitrile-, butylated hydroxyanisole-, trans-stilbene oxide-inducibility of hepatic GST activities in the same species towards different substrates. 3. For example, in rats GST activities for EA, DCNB and TPBO increased respectively, 2.3-, 4.8- and 25-fold during age-development, and after treatment with TSO 1.2-, 3.6- and 1.3-fold. 4. Species differences were found (a) in the maturation and (b) in the inducibility of GST activities. For instance, GST activity toward EA at birth is mature in guinea pigs but not in the other species; phenobarbital treatment increased GST activities in mice and rats but not in rabbits and guinea-pigs; treatment with trans-stilbene oxide enhanced GST activity for TPBO 4.5-fold in mice but not at all in rats. 5. It is concluded that hepatic glutathione conjugation exhibits functional heterogeneity which may be due to species dependent variations in the responsiveness of GST isoenzymes to endogenous and exogenous influences.",
author = "Z. Gregus and F. Varga and A. Schmel{\'a}s",
year = "1985",
doi = "10.1016/0742-8413(85)90135-5",
language = "English",
volume = "80",
pages = "85--90",
journal = "Comparative biochemistry and physiology. C: Comparative pharmacology",
issn = "0742-8413",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Age-development and inducibility of hepatic glutathione S-transferase activities in mice, rats, rabbits and guinea-pigs

AU - Gregus, Z.

AU - Varga, F.

AU - Schmelás, A.

PY - 1985

Y1 - 1985

N2 - 1. Hepatic glutathione S-transferase (GST) activities towards l-chloro-3,4-dinitrobenzene (DNCB), 3,4-dichloronitrobenzene (DCNB), sulfobromophthalein (BSP), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), trans-4-phenyl-3-buten-2-one (TPBO) and 1,2-epoxy-3-(p-nitrophenoxy)propane (ENPP) were determined in mice, rats, rabbits and guinea-pigs during ageing and after pretreatment with enzyme inducers. 2. Variations were observed (a) in the developmental patterns and (b) in the phenobarbital-, benzo(a)pyrene-, pregnenolone-16α-carbonitrile-, butylated hydroxyanisole-, trans-stilbene oxide-inducibility of hepatic GST activities in the same species towards different substrates. 3. For example, in rats GST activities for EA, DCNB and TPBO increased respectively, 2.3-, 4.8- and 25-fold during age-development, and after treatment with TSO 1.2-, 3.6- and 1.3-fold. 4. Species differences were found (a) in the maturation and (b) in the inducibility of GST activities. For instance, GST activity toward EA at birth is mature in guinea pigs but not in the other species; phenobarbital treatment increased GST activities in mice and rats but not in rabbits and guinea-pigs; treatment with trans-stilbene oxide enhanced GST activity for TPBO 4.5-fold in mice but not at all in rats. 5. It is concluded that hepatic glutathione conjugation exhibits functional heterogeneity which may be due to species dependent variations in the responsiveness of GST isoenzymes to endogenous and exogenous influences.

AB - 1. Hepatic glutathione S-transferase (GST) activities towards l-chloro-3,4-dinitrobenzene (DNCB), 3,4-dichloronitrobenzene (DCNB), sulfobromophthalein (BSP), p-nitrobenzyl chloride (NBC), ethacrynic acid (EA), trans-4-phenyl-3-buten-2-one (TPBO) and 1,2-epoxy-3-(p-nitrophenoxy)propane (ENPP) were determined in mice, rats, rabbits and guinea-pigs during ageing and after pretreatment with enzyme inducers. 2. Variations were observed (a) in the developmental patterns and (b) in the phenobarbital-, benzo(a)pyrene-, pregnenolone-16α-carbonitrile-, butylated hydroxyanisole-, trans-stilbene oxide-inducibility of hepatic GST activities in the same species towards different substrates. 3. For example, in rats GST activities for EA, DCNB and TPBO increased respectively, 2.3-, 4.8- and 25-fold during age-development, and after treatment with TSO 1.2-, 3.6- and 1.3-fold. 4. Species differences were found (a) in the maturation and (b) in the inducibility of GST activities. For instance, GST activity toward EA at birth is mature in guinea pigs but not in the other species; phenobarbital treatment increased GST activities in mice and rats but not in rabbits and guinea-pigs; treatment with trans-stilbene oxide enhanced GST activity for TPBO 4.5-fold in mice but not at all in rats. 5. It is concluded that hepatic glutathione conjugation exhibits functional heterogeneity which may be due to species dependent variations in the responsiveness of GST isoenzymes to endogenous and exogenous influences.

UR - http://www.scopus.com/inward/record.url?scp=0021998187&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021998187&partnerID=8YFLogxK

U2 - 10.1016/0742-8413(85)90135-5

DO - 10.1016/0742-8413(85)90135-5

M3 - Article

VL - 80

SP - 85

EP - 90

JO - Comparative biochemistry and physiology. C: Comparative pharmacology

JF - Comparative biochemistry and physiology. C: Comparative pharmacology

SN - 0742-8413

IS - 1

ER -