Age determines the magnitudes of angiotensin II-induced contractions, mRNA, and protein expression of angiotensin type 1 receptors in rat carotid arteries

Zoltan Vamos, Peter Cseplo, Ivan Ivic, Robert Matics, J. Hamar, Akos Koller

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In this study, we hypothesized that aging alters angiotensin II (Ang II)-induced vasomotor responses and expression of vascular mRNA and protein angiotensin type 1 receptor (AT1R). Thus, carotid arteries were isolated from the following age groups of rats: 8 days, 2-9 months, 12-20 months, and 20-30 months, and their vasomotor responses were measured in a myograph after repeated administrations of Ang II. Vascular relative AT 1R mRNA level was determined by quantitative reverse-transcriptase polymerase chain reaction and the AT1R protein density was measured by Western blot. Contractions to the first administration of Ang II increased from 8 days to 6 months and then they decreased to 30 months. In general, second administration of Ang II elicited reduced contractions, but they also increased from 8 days until 2 months and then they decreased to 30 months. Similarly the AT1R mRNA level increased from 8 days to 12 months and then decreased to 30 months. Similarly the AT1R protein density increased from 8 days until 16 months and then they decreased to 30 months. The pattern of these changes correlated with functional vasomotor data. We conclude that aging (newborn to senescence) has substantial effects on Ang II-induced vasomotor responses and AT1R signaling suggesting the importance of genetic programs.

Original languageEnglish
Pages (from-to)519-526
Number of pages8
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume69 A
Issue number5
DOIs
Publication statusPublished - 2014

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Angiotensin Type 1 Receptor
Carotid Arteries
Angiotensin II
Messenger RNA
Proteins
Blood Vessels
Reverse Transcriptase Polymerase Chain Reaction
Age Groups
Western Blotting
Newborn Infant

Keywords

  • Aging
  • Angiotensin II-induced arterial responses
  • AT1R mRNA expression
  • AT1R protein expression
  • Senescence.

ASJC Scopus subject areas

  • Ageing
  • Geriatrics and Gerontology
  • Medicine(all)

Cite this

Age determines the magnitudes of angiotensin II-induced contractions, mRNA, and protein expression of angiotensin type 1 receptors in rat carotid arteries. / Vamos, Zoltan; Cseplo, Peter; Ivic, Ivan; Matics, Robert; Hamar, J.; Koller, Akos.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 69 A, No. 5, 2014, p. 519-526.

Research output: Contribution to journalArticle

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