Age-Dependent Variations of Intralysosomal Enzyme Release from Human PMN Leukocytes under Various Stimuli

T. Fülöp, I. Komáromi, Gabriella Fóris, I. Wórum, A. Leövey

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The intralysosomal β glucuronidase and elastase release from polymorphonuclear leukocytes (PMNL) of young and aged male subjects were determined after 60-min incubation with 10 μg/ml Cytochalasin B (CB), 10-6 M of Ca ionophore A 23187 and various concentrations of human low density lipoprotein (LDL). The γ glucoronidase secretion was triggered by both A 23187 and LDL; however, no significant differences were found between the enzyme release from PMNLs of young and aged subjects. In contrast, a marked elastase release was triggered in the young group only by LDL, whereas in the aged group, all of the applied drugs induced a significant elastase release. LDL caused the most dramatic enzyme release from PMNLs of aged males. It was concluded that the release of PMNL-elastase after LDL incorporation as well as by CB and Ca ionophore stimulation may be an age-related process.

Original languageEnglish
Pages (from-to)302-310
Number of pages9
JournalImmunobiology
Volume171
Issue number3
DOIs
Publication statusPublished - Jan 1 1986

Keywords

  • A 23187
  • CB
  • Ca ionophore A 23187
  • Cytochalasin B
  • DMSO
  • Dimethyl-sulfoxide
  • LDL
  • Low-Density lipoprotein
  • PMNL
  • Polymorphonuclear leukocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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