Age-dependent oxidative stress-induced DNA damage in Down's lymphocytes

Marianna Zana, Anita Szécsényi, Ágnes Czibula, Annamária Bjelik, Anna Juhász, Ágnes Rimanóczy, Krisztina Szabó, Ágnes Vetró, Péter Szucs, Ágnes Várkonyi, Magdolna Pákáski, Krisztina Boda, István Raskó, Zoltán Janka, János Kálmán

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33 Citations (Scopus)

Abstract

The aim of the present study was to investigate the oxidative status of lymphocytes from children (n = 7) and adults (n = 18) with Down's syndrome (DS). The basal oxidative condition, the vulnerability to in vitro hydrogen peroxide exposure, and the repair capacity were measured by means of the damage-specific alkaline comet assay. Significantly and age-independently elevated numbers of single strand breaks and oxidized bases (pyrimidines and purines) were found in the nuclear DNA of the lymphocytes in the DS group in the basal condition. These results may support the role of an increased level of endogenous oxidative stress in DS and are similar to those previously demonstrated in Alzheimer's disease. In the in vitro oxidative stress-induced state, a markedly higher extent of DNA damage was observed in DS children as compared with age- and gender-matched healthy controls, suggesting that young trisomic lymphocytes are more sensitive to oxidative stress than normal ones. However, the repair ability itself was not found to be deteriorated in either DS children or DS adults.

Original languageEnglish
Pages (from-to)726-733
Number of pages8
JournalBiochemical and biophysical research communications
Volume345
Issue number2
DOIs
Publication statusPublished - Jun 30 2006

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Keywords

  • Alzheimer's disease
  • Comet assay
  • DNA repair
  • Down's syndrome
  • Lymphocyte
  • Oxidative stress

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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