Age-dependent influence of octreotide on stimulated pancreatic growth in the postnatal period of rats

Krisztina Kisfalvi, Helmut Friess, Markus W. Büchler, Zsolt Tulassay, Gabor Varsa, Miklós Papp

Research output: Contribution to journalArticle

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Abstract

Objective: To investigate the effect of long-acting octreotide (SMS 201-995) on the plasma growth hormone level in preweaning rats and to study the growth and composition of the exocrine pancreas in these rats after stimulation by caerulein- and camostate-induced endogenous cholecystokinin (CCK). Methods: Wistar rats of both sexes were treated as littermate pairs in two periods of postnatal age, from days 1 to 11 and from days 11 to 21. To stimulate pancreatic growth, caerulein (3 μg/kg subcutaneously three times daily) was given from days 1 to 11, and oral camostate (200 mg/kg given once daily) or CCK-8 (10 μg/kg subcutaneously three times daily) was administered from days 11 to 21. Octreotide (6 or 15 μg/kg subcutaneously twice daily) was administered alone or in combination with caerulein or camostate. The rats were exsanguinated on days 11 or 21, and each pancreas was removed, weighed and analysed. Results: Caerulein stimulated pancreatic growth and raised the trypsin concentration; camostate induced pancreatic hypertrophy and hyperplasia. By day 11, octreotide had decreased the plasma growth hormone level and the basal pancreatic trypsin concentration and content. Given in combination with caerulein, octreotide reduced the growth hormone level and the stimulated trypsin and DNA contents. By day 21, rats treated with octreotide in the camostate group showed a reduced basal pancreatic trypsin concentration and a decreased basal trypsin content (although the changes were not significant). Plasma growth hormone levels were not significantly reduced. Conclusion: The antitrophic pancreatic action of octreotide and its plasma growth hormone-lowering effect were shown in rats during the first 10 days after birth. These effects were less notable from days 11 to 21, a period when CCK receptors increase in number and components of the stimulus-secretion mechanism are mature.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalEuropean Journal of Gastroenterology and Hepatology
Volume8
Issue number1
Publication statusPublished - 1996

Fingerprint

Octreotide
Ceruletide
Trypsin
Growth Hormone
Growth
Cholecystokinin Receptors
Exocrine Pancreas
Cholecystokinin
Hypertrophy
Hyperplasia
Wistar Rats
Pancreas
camostat
Parturition
DNA

Keywords

  • Caerulein
  • Camostate
  • CCK-8
  • Growth hormone
  • Long-acting somatostatin
  • Pancreatic growth
  • Preweaning rat

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Age-dependent influence of octreotide on stimulated pancreatic growth in the postnatal period of rats. / Kisfalvi, Krisztina; Friess, Helmut; Büchler, Markus W.; Tulassay, Zsolt; Varsa, Gabor; Papp, Miklós.

In: European Journal of Gastroenterology and Hepatology, Vol. 8, No. 1, 1996, p. 69-74.

Research output: Contribution to journalArticle

Kisfalvi, Krisztina ; Friess, Helmut ; Büchler, Markus W. ; Tulassay, Zsolt ; Varsa, Gabor ; Papp, Miklós. / Age-dependent influence of octreotide on stimulated pancreatic growth in the postnatal period of rats. In: European Journal of Gastroenterology and Hepatology. 1996 ; Vol. 8, No. 1. pp. 69-74.
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AU - Kisfalvi, Krisztina

AU - Friess, Helmut

AU - Büchler, Markus W.

AU - Tulassay, Zsolt

AU - Varsa, Gabor

AU - Papp, Miklós

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AB - Objective: To investigate the effect of long-acting octreotide (SMS 201-995) on the plasma growth hormone level in preweaning rats and to study the growth and composition of the exocrine pancreas in these rats after stimulation by caerulein- and camostate-induced endogenous cholecystokinin (CCK). Methods: Wistar rats of both sexes were treated as littermate pairs in two periods of postnatal age, from days 1 to 11 and from days 11 to 21. To stimulate pancreatic growth, caerulein (3 μg/kg subcutaneously three times daily) was given from days 1 to 11, and oral camostate (200 mg/kg given once daily) or CCK-8 (10 μg/kg subcutaneously three times daily) was administered from days 11 to 21. Octreotide (6 or 15 μg/kg subcutaneously twice daily) was administered alone or in combination with caerulein or camostate. The rats were exsanguinated on days 11 or 21, and each pancreas was removed, weighed and analysed. Results: Caerulein stimulated pancreatic growth and raised the trypsin concentration; camostate induced pancreatic hypertrophy and hyperplasia. By day 11, octreotide had decreased the plasma growth hormone level and the basal pancreatic trypsin concentration and content. Given in combination with caerulein, octreotide reduced the growth hormone level and the stimulated trypsin and DNA contents. By day 21, rats treated with octreotide in the camostate group showed a reduced basal pancreatic trypsin concentration and a decreased basal trypsin content (although the changes were not significant). Plasma growth hormone levels were not significantly reduced. Conclusion: The antitrophic pancreatic action of octreotide and its plasma growth hormone-lowering effect were shown in rats during the first 10 days after birth. These effects were less notable from days 11 to 21, a period when CCK receptors increase in number and components of the stimulus-secretion mechanism are mature.

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KW - Pancreatic growth

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