Age-dependent changes of K-elastin stimulated effector functions of human phagocytic cells: Relevance for atherogenesis

Z. Varga, M. P. Jacob, L. Robert, J. Csongor, T. Fulop

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Effector functions of the elastin receptor on human phagocytic cells from young and older individuals were studied. In cells of young healthy subjects the elastin peptides, the agonists of receptor, stimulated both superoxide anion release from PMNs and phagocytosis of coated human red cells by monocytes. Elastin appeared to inhibit the cholesterol synthesis in monocytes, measured by the incorporation of 14C-acetate. In comparison with phagocytic cells of young (≤25 ± 6 years) subjects, PMNs of elderly donors (≤75 ± 10 years) bore a similar number of binding sites for soluble elastin peptides, and the affinity of the elastin receptor was unchanged as shown by Scatchard analysis. The phagocytosis of coated human red cells stimulated by elastin peptides was also similar in the two age groups. However, several differences were found between phagocytic cells of young and elderly donors 1) PMNs of elderly released increased amounts of elastase from both resting and elastin peptide stimulated cells, and 2) monocytes of elderly showed a lack of inhibition of cholesterol synthesis by elastin peptides when maintained in cholesterol-free medium. These changes in effector functions of phagocytic cells from elderly donors might contribute to the age-dependent increase of susceptibility to the development of atherosclerotic lesions.

Original languageEnglish
Pages (from-to)653-662
Number of pages10
JournalExperimental Gerontology
Volume32
Issue number6
DOIs
Publication statusPublished - Nov 1997

Fingerprint

Elastin
Phagocytes
Atherosclerosis
Peptides
Monocytes
Cholesterol
Tissue Donors
Phagocytosis
Cells
Peptide Receptors
Pancreatic Elastase
Superoxides
Healthy Volunteers
Acetates
Age Groups
Binding Sites

Keywords

  • Aging
  • Atherogenesis
  • Cholesterol synthesis
  • Elastase activity
  • Elastin receptor
  • Phagocytic cells
  • Phagocytosis
  • Respiratory burst
  • Superoxide production

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

Age-dependent changes of K-elastin stimulated effector functions of human phagocytic cells : Relevance for atherogenesis. / Varga, Z.; Jacob, M. P.; Robert, L.; Csongor, J.; Fulop, T.

In: Experimental Gerontology, Vol. 32, No. 6, 11.1997, p. 653-662.

Research output: Contribution to journalArticle

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