Age-dependent changes in transmembrane signalling: Identification of G proteins in human lymphocytes and polymorphonuclear leukocytes

Tamás Fulop, György Barabas, Z. Varga, Csongor József, Sándor Csabina, Sándor Szucs, I. Seres, Edit Szikszay, Zsolt Jeney, András Penyige

Research output: Contribution to journalArticle

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Abstract

In human neutrophils (PMNLs) we found that in the elderly IP3 formation was significantly decreased compared to that of young subjects. For FMLP receptor binding affinity and number no measurable differences occurred upon ageing, studying both the low or the high affinity receptors. The amount of ADP-ribosylated G proteins, catalysed by pertussis toxin (PT) or cholera toxin (CT), was significantly increased in PMNLs of the elderly. In lymphocytes, the PT-catalysed ADP ribosylation of G proteins was also increased with ageing, while the CT-catalysed ribosylation was decreased. The autoradiogram of [32P]ADP-ribosylated proteins by CT in lymphocytes of young individuals showed a major polypeptide of 40,000 Mr. In contrast, in lymphocytes of the elderly, the major polypeptide was 45,000 Mr. In PMNLs, CT labelled quite strongly the 45,000 Mr band, mainly in the elderly. When PT was used, no age-related pattern changes could be demonstrated, while differences could be observed between the two types of cells. The use of antiserum P680 (Grmαcommon showed no age-related pattern changes, while the intensity of the labelled proteins varies with age and cell type. The antiserum U46 (G) could identify in lymphocytes of young subjects two polypeptides 68,000 and 41,000Mr. The prominent polypeptide in lymphocytes of the elderly was the 70,000Mr, and no other polypeptides could be recognized. In PMNLs of young subjects the U46 and serum identified a range of species. In PMNLs of the elderly all these bands were weakly labelled. The present data indicate changes in the pattern and the quantity of G proteins in lymphocytes and PMNLs of elderly subjects.

Original languageEnglish
Pages (from-to)593-603
Number of pages11
JournalCellular Signalling
Volume5
Issue number5
DOIs
Publication statusPublished - 1993

Fingerprint

GTP-Binding Proteins
Neutrophils
Lymphocytes
Cholera Toxin
Pertussis Toxin
Peptides
Adenosine Diphosphate
Immune Sera
Formyl Peptide Receptor
Proteins
Serum

Keywords

  • ageing
  • FMLP receptor
  • G proteins
  • granulocytes
  • lymphocytes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Age-dependent changes in transmembrane signalling : Identification of G proteins in human lymphocytes and polymorphonuclear leukocytes. / Fulop, Tamás; Barabas, György; Varga, Z.; József, Csongor; Csabina, Sándor; Szucs, Sándor; Seres, I.; Szikszay, Edit; Jeney, Zsolt; Penyige, András.

In: Cellular Signalling, Vol. 5, No. 5, 1993, p. 593-603.

Research output: Contribution to journalArticle

Fulop, Tamás ; Barabas, György ; Varga, Z. ; József, Csongor ; Csabina, Sándor ; Szucs, Sándor ; Seres, I. ; Szikszay, Edit ; Jeney, Zsolt ; Penyige, András. / Age-dependent changes in transmembrane signalling : Identification of G proteins in human lymphocytes and polymorphonuclear leukocytes. In: Cellular Signalling. 1993 ; Vol. 5, No. 5. pp. 593-603.
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T2 - Identification of G proteins in human lymphocytes and polymorphonuclear leukocytes

AU - Fulop, Tamás

AU - Barabas, György

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AU - József, Csongor

AU - Csabina, Sándor

AU - Szucs, Sándor

AU - Seres, I.

AU - Szikszay, Edit

AU - Jeney, Zsolt

AU - Penyige, András

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AB - In human neutrophils (PMNLs) we found that in the elderly IP3 formation was significantly decreased compared to that of young subjects. For FMLP receptor binding affinity and number no measurable differences occurred upon ageing, studying both the low or the high affinity receptors. The amount of ADP-ribosylated G proteins, catalysed by pertussis toxin (PT) or cholera toxin (CT), was significantly increased in PMNLs of the elderly. In lymphocytes, the PT-catalysed ADP ribosylation of G proteins was also increased with ageing, while the CT-catalysed ribosylation was decreased. The autoradiogram of [32P]ADP-ribosylated proteins by CT in lymphocytes of young individuals showed a major polypeptide of 40,000 Mr. In contrast, in lymphocytes of the elderly, the major polypeptide was 45,000 Mr. In PMNLs, CT labelled quite strongly the 45,000 Mr band, mainly in the elderly. When PT was used, no age-related pattern changes could be demonstrated, while differences could be observed between the two types of cells. The use of antiserum P680 (Grmαcommon showed no age-related pattern changes, while the intensity of the labelled proteins varies with age and cell type. The antiserum U46 (Goα) could identify in lymphocytes of young subjects two polypeptides 68,000 and 41,000Mr. The prominent polypeptide in lymphocytes of the elderly was the 70,000Mr, and no other polypeptides could be recognized. In PMNLs of young subjects the U46 and serum identified a range of species. In PMNLs of the elderly all these bands were weakly labelled. The present data indicate changes in the pattern and the quantity of G proteins in lymphocytes and PMNLs of elderly subjects.

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