Age-dependent changes at the blood-brain barrier. A Comparative structural and functional study in young adult and middle aged rats

Luca Bors, Kinga Tóth, Estilla Zsófia Tóth, Ágnes Bajza, Attila Csorba, Krisztián Szigeti, Domokos Máthé, Gábor Perlaki, Gergely Orsi, G. Tóth, Franciska Erdő

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Decreased beta-amyloid clearance in Alzheimer's disease and increased blood-brain barrier permeability in aged subjects have been reported in several articles. However, morphological and functional characterization of blood-brain barrier and its membrane transporter activity have not been described in physiological aging yet. The aim of our study was to explore the structural changes in the brain microvessels and possible functional alterations of P-glycoprotein at the blood-brain barrier with aging. Our approach included MR imaging for anatomical orientation in middle aged rats, electronmicroscopy and immunohistochemistry to analyse the alterations at cellular level, dual or triple-probe microdialysis and SPECT to test P-glycoprotein functionality in young and middle aged rats. Our results indicate that the thickness of basal lamina increases, the number of tight junctions decreases and the size of astrocyte endfeet extends with advanced age. On the basis of microdialysis and SPECT results the P-gp function is reduced in old rats. With our multiparametric approach a complex regulation can be suggested which includes elements leading to increased permeability of blood-brain barrier by enhanced paracellular and transcellular transport, and factors working against it. To verify the role of P-gp pumps in brain aging further studies are warranted.

Original languageEnglish
Pages (from-to)269-277
Number of pages9
JournalBrain Research Bulletin
Volume139
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Blood-Brain Barrier
Young Adult
Microdialysis
P-Glycoprotein
Single-Photon Emission-Computed Tomography
Permeability
Transcytosis
Membrane Transport Proteins
Tight Junctions
Brain
Microvessels
Amyloid
Basement Membrane
Astrocytes
Alzheimer Disease
Immunohistochemistry

Keywords

  • Aging
  • Blood-brain barrier
  • Imaging
  • Microdialysis
  • P-Glycoprotein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Age-dependent changes at the blood-brain barrier. A Comparative structural and functional study in young adult and middle aged rats. / Bors, Luca; Tóth, Kinga; Tóth, Estilla Zsófia; Bajza, Ágnes; Csorba, Attila; Szigeti, Krisztián; Máthé, Domokos; Perlaki, Gábor; Orsi, Gergely; Tóth, G.; Erdő, Franciska.

In: Brain Research Bulletin, Vol. 139, 01.05.2018, p. 269-277.

Research output: Contribution to journalArticle

Bors, Luca ; Tóth, Kinga ; Tóth, Estilla Zsófia ; Bajza, Ágnes ; Csorba, Attila ; Szigeti, Krisztián ; Máthé, Domokos ; Perlaki, Gábor ; Orsi, Gergely ; Tóth, G. ; Erdő, Franciska. / Age-dependent changes at the blood-brain barrier. A Comparative structural and functional study in young adult and middle aged rats. In: Brain Research Bulletin. 2018 ; Vol. 139. pp. 269-277.
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