Age-dependent alterations of human recombinant GM-CSF effects on human granulocytes

Ildikó Seres, József Csongor, Attila Mohácsi, András Leövey, Tamás Fülöp

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The granulocyte-macrophage colony stimulating (GM-CSF) is an important in vivo regulator of granulopoiesis and neutrophil functions. It is well-known that the immune response and the transmembrane signalling in immune cells change with aging. We wished to elucidate the effects of GM-CSF in itself and in priming the activities of other inflammatory agents on neutrophils of elderly persons. Neutrophils of 20 healthy elderly (aged 60-90 years) and 20 healthy young (aged 20-25 years) subjects were studied for superoxide anion production, intracellular free calcium mobilization, antibody dependent cellular cytotoxicity (ADCC) and intracellular killing activities. It was found that GM-CSF is unable to prime neutrophils of elderly subjects to the action of FMLP, metenkephalin or opsonized symosan. By the use of Pertussis toxin and H7 it was demonstrated that a different signal transduction pathway in neutrophils of elderly subjects is activated by GM-CSF or FMLP if compared to that of young subjects. These results suggest that the lack of priming could contribute to the greater susceptibility of the elderly to infections and that the change of the signal transduction mechanism in neutrophils of elderly subjects might partly explain this phenomenon.

Original languageEnglish
Pages (from-to)143-154
Number of pages12
JournalMechanisms of Ageing and Development
Volume71
Issue number1-2
DOIs
Publication statusPublished - Oct 1 1993

Keywords

  • ADCC
  • Aging
  • GM-CSF
  • Intracellular killing
  • Neutrophils
  • Signal transduction

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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