AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro

Gábor Gyulai, Mervyn Humphreys, András Bittsánszky, Kirsten Skøt, J. Kiss, Leif Skøt, G. Gullner, Sue Heywood, Zoltán Szabó, Alan Lovatt, László Radimszky, Hywel Roderick, Heinz Rennenberg, Michael Abberton, T. Kőmíves, L. Heszky

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10 -1 to 10-5 M) incubated for 21 days on aseptic tissue culture media WPM containing 1 μM Cu. Zn2+ caused phytotoxicity only at high concentrations (10-1 to 10-2 M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.

Original languageEnglish
Pages (from-to)300-306
Number of pages7
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume60
Issue number3-4
Publication statusPublished - Mar 2005

Fingerprint

Populus canescens
Amplified Fragment Length Polymorphism Analysis
Populus
phytoremediation
amplified fragment length polymorphism
Copper
Clone Cells
copper
genetically modified organisms
clones
Tissue culture
Heavy Metals
Polymorphism
Culture Media
Zinc
Soils
DNA
Environmental Biodegradation
Mutation Rate
phytotoxicity

Keywords

  • chl-ECS (lgl6)
  • cyt-ECS (ggs11)
  • Phytoextraction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro. / Gyulai, Gábor; Humphreys, Mervyn; Bittsánszky, András; Skøt, Kirsten; Kiss, J.; Skøt, Leif; Gullner, G.; Heywood, Sue; Szabó, Zoltán; Lovatt, Alan; Radimszky, László; Roderick, Hywel; Rennenberg, Heinz; Abberton, Michael; Kőmíves, T.; Heszky, L.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 60, No. 3-4, 03.2005, p. 300-306.

Research output: Contribution to journalArticle

Gyulai, G, Humphreys, M, Bittsánszky, A, Skøt, K, Kiss, J, Skøt, L, Gullner, G, Heywood, S, Szabó, Z, Lovatt, A, Radimszky, L, Roderick, H, Rennenberg, H, Abberton, M, Kőmíves, T & Heszky, L 2005, 'AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro', Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 60, no. 3-4, pp. 300-306.
Gyulai, Gábor ; Humphreys, Mervyn ; Bittsánszky, András ; Skøt, Kirsten ; Kiss, J. ; Skøt, Leif ; Gullner, G. ; Heywood, Sue ; Szabó, Zoltán ; Lovatt, Alan ; Radimszky, László ; Roderick, Hywel ; Rennenberg, Heinz ; Abberton, Michael ; Kőmíves, T. ; Heszky, L. / AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens L.) for copper in vitro. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2005 ; Vol. 60, No. 3-4. pp. 300-306.
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AU - Gyulai, Gábor

AU - Humphreys, Mervyn

AU - Bittsánszky, András

AU - Skøt, Kirsten

AU - Kiss, J.

AU - Skøt, Leif

AU - Gullner, G.

AU - Heywood, Sue

AU - Szabó, Zoltán

AU - Lovatt, Alan

AU - Radimszky, László

AU - Roderick, Hywel

AU - Rennenberg, Heinz

AU - Abberton, Michael

AU - Kőmíves, T.

AU - Heszky, L.

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N2 - Clone stability and in vitro phytoextraction capacity of vegetative clones of P. x canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRI-MseI digestion. Four fragments generated by EcoAGT-MseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. x canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10 -1 to 10-5 M) incubated for 21 days on aseptic tissue culture media WPM containing 1 μM Cu. Zn2+ caused phytotoxicity only at high concentrations (10-1 to 10-2 M). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper.

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