Affinity labeling of opioid receptors with enkephalin analogues

A. Borsodi, H. A. Oktem, S. Benyhe, E. Varga, J. Moitra, J. Takacs

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Chloromethyl ketone derivatives of enkephalins and Tyr-D-Ala-Gly(Me)Phe-Gly-ol (DAGO) were synthesized in normal and in tritiated form and were shown to react irreversibly at opioid receptors. Affinity of the chloromethyl ketone derivatives toward the μ site is greater than that of the parent compounds. It was shown in several studies (including autoradiography) with these ligands that the μ receptor protein contains only one type of binding subunit whose apparent M(r) is 58000.

Original languageEnglish
Title of host publicationNew leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914
EditorsJ.M. Van Ree, A.H. Mulder, V.M. Wiegant, T.B. Van Wimersma Greidanus, J.M. Van Ree, A.H. Mulder, V.M. Wiegant, T.B. Van Wimersma Greidanus
PublisherElsevier Science Publishers B.V.
Pages171
Number of pages1
ISBN (Print)044481213X
Publication statusPublished - 1990
EventThe International Narcotics Research Conference -
Duration: Jul 8 1989Jul 13 1989

Other

OtherThe International Narcotics Research Conference
Period7/8/897/13/89

Fingerprint

Enkephalins
Opioid Receptors
Ketones
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Autoradiography
Ligands
Proteins
tyrosyl-alanyl-glycyl-phenylalanine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Borsodi, A., Oktem, H. A., Benyhe, S., Varga, E., Moitra, J., & Takacs, J. (1990). Affinity labeling of opioid receptors with enkephalin analogues. In J. M. Van Ree, A. H. Mulder, V. M. Wiegant, T. B. Van Wimersma Greidanus, J. M. Van Ree, A. H. Mulder, V. M. Wiegant, ... T. B. Van Wimersma Greidanus (Eds.), New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914 (pp. 171). Elsevier Science Publishers B.V..

Affinity labeling of opioid receptors with enkephalin analogues. / Borsodi, A.; Oktem, H. A.; Benyhe, S.; Varga, E.; Moitra, J.; Takacs, J.

New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914. ed. / J.M. Van Ree; A.H. Mulder; V.M. Wiegant; T.B. Van Wimersma Greidanus; J.M. Van Ree; A.H. Mulder; V.M. Wiegant; T.B. Van Wimersma Greidanus. Elsevier Science Publishers B.V., 1990. p. 171.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Borsodi, A, Oktem, HA, Benyhe, S, Varga, E, Moitra, J & Takacs, J 1990, Affinity labeling of opioid receptors with enkephalin analogues. in JM Van Ree, AH Mulder, VM Wiegant, TB Van Wimersma Greidanus, JM Van Ree, AH Mulder, VM Wiegant & TB Van Wimersma Greidanus (eds), New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914. Elsevier Science Publishers B.V., pp. 171, The International Narcotics Research Conference, 7/8/89.
Borsodi A, Oktem HA, Benyhe S, Varga E, Moitra J, Takacs J. Affinity labeling of opioid receptors with enkephalin analogues. In Van Ree JM, Mulder AH, Wiegant VM, Van Wimersma Greidanus TB, Van Ree JM, Mulder AH, Wiegant VM, Van Wimersma Greidanus TB, editors, New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914. Elsevier Science Publishers B.V. 1990. p. 171
Borsodi, A. ; Oktem, H. A. ; Benyhe, S. ; Varga, E. ; Moitra, J. ; Takacs, J. / Affinity labeling of opioid receptors with enkephalin analogues. New leads in opioid research: proceedings of the International Narcotics Research Conference. ICS914. editor / J.M. Van Ree ; A.H. Mulder ; V.M. Wiegant ; T.B. Van Wimersma Greidanus ; J.M. Van Ree ; A.H. Mulder ; V.M. Wiegant ; T.B. Van Wimersma Greidanus. Elsevier Science Publishers B.V., 1990. pp. 171
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