Advances in renal (patho)physiology using multiphoton microscopy

A. Sipos, I. Toma, J. J. Kang, L. Rosivall, J. Peti-Peterdi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Multiphoton excitation fluorescence microscopy is a state-of-the-art confocal imaging technique ideal for deep optical sectioning of living tissues. It is capable of performing ultrasensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution which other imaging modalities cannot achieve. For more than a decade, multiphoton microscopy has been successfully used with various in vitro and in vivo experimental approaches to study many functions of different organs, including the kidney. This study focuses on recent advances in our knowledge of renal (patho)physiological processes made possible by the use of this imaging technology. Visualization of cellular variables like cytosolic calcium, pH, cell-to-cell communication and signal propagation, interstitial fluid flow in the juxtaglomerular apparatus (JGA), real-time imaging of tubuloglomerular feedback (TGF), and renin release mechanisms are reviewed. A brief summary is provided of kidney functions that can be measured by in vivo quantitative multiphoton imaging including glomerular filtration and permeability, concentration, dilution, and activity of the intrarenal renin-angiotensin system using this minimally invasive approach. New visual data challenge a number of existing paradigms in renal (patho)physiology. Also, quantitative imaging of kidney function with multiphoton microscopy has tremendous potential to eventually provide novel non-invasive diagnostic and therapeutic tools for future applications in clinical nephrology.

Original languageEnglish
Pages (from-to)1188-1191
Number of pages4
JournalKidney International
Volume72
Issue number10
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Microscopy
Kidney
Multiphoton Fluorescence Microscopy
Juxtaglomerular Apparatus
Physiological Phenomena
Nephrology
Extracellular Fluid
Renin-Angiotensin System
Renin
Cell Communication
Permeability
Calcium
Technology
Health
Therapeutics

Keywords

  • Pathophysiology of renal disease and progression
  • Renal hemodynamics
  • Renal morphology
  • Renin angiotensin system
  • Tubular epithelium

ASJC Scopus subject areas

  • Nephrology

Cite this

Advances in renal (patho)physiology using multiphoton microscopy. / Sipos, A.; Toma, I.; Kang, J. J.; Rosivall, L.; Peti-Peterdi, J.

In: Kidney International, Vol. 72, No. 10, 11.2007, p. 1188-1191.

Research output: Contribution to journalArticle

Sipos, A, Toma, I, Kang, JJ, Rosivall, L & Peti-Peterdi, J 2007, 'Advances in renal (patho)physiology using multiphoton microscopy', Kidney International, vol. 72, no. 10, pp. 1188-1191. https://doi.org/10.1038/sj.ki.5002461
Sipos, A. ; Toma, I. ; Kang, J. J. ; Rosivall, L. ; Peti-Peterdi, J. / Advances in renal (patho)physiology using multiphoton microscopy. In: Kidney International. 2007 ; Vol. 72, No. 10. pp. 1188-1191.
@article{25dd0bf031894888ab8963953e0c6230,
title = "Advances in renal (patho)physiology using multiphoton microscopy",
abstract = "Multiphoton excitation fluorescence microscopy is a state-of-the-art confocal imaging technique ideal for deep optical sectioning of living tissues. It is capable of performing ultrasensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution which other imaging modalities cannot achieve. For more than a decade, multiphoton microscopy has been successfully used with various in vitro and in vivo experimental approaches to study many functions of different organs, including the kidney. This study focuses on recent advances in our knowledge of renal (patho)physiological processes made possible by the use of this imaging technology. Visualization of cellular variables like cytosolic calcium, pH, cell-to-cell communication and signal propagation, interstitial fluid flow in the juxtaglomerular apparatus (JGA), real-time imaging of tubuloglomerular feedback (TGF), and renin release mechanisms are reviewed. A brief summary is provided of kidney functions that can be measured by in vivo quantitative multiphoton imaging including glomerular filtration and permeability, concentration, dilution, and activity of the intrarenal renin-angiotensin system using this minimally invasive approach. New visual data challenge a number of existing paradigms in renal (patho)physiology. Also, quantitative imaging of kidney function with multiphoton microscopy has tremendous potential to eventually provide novel non-invasive diagnostic and therapeutic tools for future applications in clinical nephrology.",
keywords = "Pathophysiology of renal disease and progression, Renal hemodynamics, Renal morphology, Renin angiotensin system, Tubular epithelium",
author = "A. Sipos and I. Toma and Kang, {J. J.} and L. Rosivall and J. Peti-Peterdi",
year = "2007",
month = "11",
doi = "10.1038/sj.ki.5002461",
language = "English",
volume = "72",
pages = "1188--1191",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Advances in renal (patho)physiology using multiphoton microscopy

AU - Sipos, A.

AU - Toma, I.

AU - Kang, J. J.

AU - Rosivall, L.

AU - Peti-Peterdi, J.

PY - 2007/11

Y1 - 2007/11

N2 - Multiphoton excitation fluorescence microscopy is a state-of-the-art confocal imaging technique ideal for deep optical sectioning of living tissues. It is capable of performing ultrasensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution which other imaging modalities cannot achieve. For more than a decade, multiphoton microscopy has been successfully used with various in vitro and in vivo experimental approaches to study many functions of different organs, including the kidney. This study focuses on recent advances in our knowledge of renal (patho)physiological processes made possible by the use of this imaging technology. Visualization of cellular variables like cytosolic calcium, pH, cell-to-cell communication and signal propagation, interstitial fluid flow in the juxtaglomerular apparatus (JGA), real-time imaging of tubuloglomerular feedback (TGF), and renin release mechanisms are reviewed. A brief summary is provided of kidney functions that can be measured by in vivo quantitative multiphoton imaging including glomerular filtration and permeability, concentration, dilution, and activity of the intrarenal renin-angiotensin system using this minimally invasive approach. New visual data challenge a number of existing paradigms in renal (patho)physiology. Also, quantitative imaging of kidney function with multiphoton microscopy has tremendous potential to eventually provide novel non-invasive diagnostic and therapeutic tools for future applications in clinical nephrology.

AB - Multiphoton excitation fluorescence microscopy is a state-of-the-art confocal imaging technique ideal for deep optical sectioning of living tissues. It is capable of performing ultrasensitive, quantitative imaging of organ functions in health and disease with high spatial and temporal resolution which other imaging modalities cannot achieve. For more than a decade, multiphoton microscopy has been successfully used with various in vitro and in vivo experimental approaches to study many functions of different organs, including the kidney. This study focuses on recent advances in our knowledge of renal (patho)physiological processes made possible by the use of this imaging technology. Visualization of cellular variables like cytosolic calcium, pH, cell-to-cell communication and signal propagation, interstitial fluid flow in the juxtaglomerular apparatus (JGA), real-time imaging of tubuloglomerular feedback (TGF), and renin release mechanisms are reviewed. A brief summary is provided of kidney functions that can be measured by in vivo quantitative multiphoton imaging including glomerular filtration and permeability, concentration, dilution, and activity of the intrarenal renin-angiotensin system using this minimally invasive approach. New visual data challenge a number of existing paradigms in renal (patho)physiology. Also, quantitative imaging of kidney function with multiphoton microscopy has tremendous potential to eventually provide novel non-invasive diagnostic and therapeutic tools for future applications in clinical nephrology.

KW - Pathophysiology of renal disease and progression

KW - Renal hemodynamics

KW - Renal morphology

KW - Renin angiotensin system

KW - Tubular epithelium

UR - http://www.scopus.com/inward/record.url?scp=35848961934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35848961934&partnerID=8YFLogxK

U2 - 10.1038/sj.ki.5002461

DO - 10.1038/sj.ki.5002461

M3 - Article

C2 - 17667980

AN - SCOPUS:35848961934

VL - 72

SP - 1188

EP - 1191

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 10

ER -