Adsorption equilibria of proline in hydrophilic interaction chromatography

Péter Vajda, Attila Felinger, Alberto Cavazzini

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The adsorption behavior of proline under hydrophilic interaction chromatography conditions was investigated from six aqueous solutions of acetonitrile. Proline adsorption isotherms were recorded at each mobile phase composition by frontal analysis and inverse method. The BET model was found to be the best choice to describe the nonlinear behavior of proline adsorption under hydrophilic interaction chromatography conditions. The adsorption isotherm parameters were derived from two independent parameter estimation methods. The parameters derived from regression analysis of the frontal analysis data and from overloaded elution bands were found to be in good agreement with the excess isotherm of water. The mobile phase composition at which the maximum excess adsorption of water was observed corresponded to the maximum saturation capacity measured for proline.

Original languageEnglish
Pages (from-to)5965-5970
Number of pages6
JournalJournal of Chromatography A
Issue number38
Publication statusPublished - Sep 1 2010



  • Equilibrium dispersive model
  • Frontal analysis
  • Proline

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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